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Microbiology > Culture and Culture Media > Flashcards

Culture and Culture Media Flashcards

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Biology 101 flashcards
1
Q

Is liquid, semi-solid or solid medium utilized to observe growth patterns or microorganism as well as for transport and storage

A

Culture Medium

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2
Q

Type of culture that is composed of only one specie

A

Pure culture

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3
Q

Type of culture that is composed of more than one species

A

Mixed culture

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4
Q

Culture that is composed of several species contained in a separate culture medium

A

Stock culture

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5
Q

Solidifying agent

A

Agar

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6
Q

A medium that contains 0% agar

A

Liquid medium

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7
Q

A semi-solid medium contains how much percent of agar? (range)

A

0.5-1%

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8
Q

A medium that contains 2-3% agar

A

Solid medium

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9
Q

Give examples of liquid medium

A

Brain Heart Infusion (BHI)
Trypticase Soy Broth (TSB)
Thioglycollate

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10
Q

Give an example of a semi-solid medium

A

Sulfide indole motility (SIM) medium

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11
Q

Give examples of a solid medium

A

Triple sugar iron (TSI) agar
Mac Conkey
Blood Agar Plate (BAP)
Chocolate agar plate (CAP)

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12
Q

What temperature will the agar melt (range)

A

80-90 degrees C

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13
Q

What temperature will the agar solidify (range)

A

40-50 degrees C

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14
Q

What is the cooling temperature for the distribution of agar into Petri plates (range)

A

55-60 degrees C

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15
Q

It is a medium where all the components are known to the user and it also used for research purposes

A

Synthetic or Defined medium

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16
Q

It is composed of some unknown substances such as peptones, meat and yeast extracts. It is very useful for isolation of bacteria.

A

Non-Synthetic or Complex medium

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17
Q

It is used for obligate intracellular bacteria

A

Tissue Culture medium

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18
Q

Examples of Non-Synthetic or Complex medium

A

Nutrient broth
Trypticase Soy Broth (TSB)
Mac Conkey agar

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19
Q

Examples of Tissue Culture Medium

A

W138
HELA 229 cells
Mc Coy cells

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20
Q

Classification of culture medium according to how it is dispensed/distributed

A

Plated medium

Tube medium

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21
Q

4 types of tube medium

A

Liquid
Slant
Butt and slant
Butt

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22
Q

Media that are routinely used in the laboratory and without added supplement. It supports the growth of most nonfastidious bacteria.

A

Simple media/ General Purpose media/ Supportive media

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23
Q

Media that is used to propagate the growth of certain group of bacteria from a mixture of organisms. It contains specific nutrients; liquid-type media.

A

Enrichment media

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24
Q

Media with added supplements such as blood, vitamins and yeast extracts necessary for the growth of fastidious organisms. These are solid-type media

A

Enriched media/ Nonselective media

25
Q

Media that allow visualization of metabolic differences between groups of bacteria

A

Differential media

26
Q

These are media incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the growth of the desired organisms

A

Selective media

27
Q

Examples of Simple media

A 
Nutrient Agar (NA) 
Nutrient Broth (NB)
Trypticase Soy Broth (TSB)
28
Q

Composition of Simple Media

A

Meat and soybean extracts

29
Q

Examples of Enrichment media

A 
Alkaline peptone water
Selenite F
Thioglycollate
Tetrathionate
Gram negative broth (GN)
Lim broth
30
Q

Examples of Enriched media

A

Blood Agar Plate (BAP)

Chocolate Agar Plate (CAP)

31
Q

It is the standard nonselective culture medium

A

Blood Agar Plate

32
Q

Used for the isolation of Hemophilus. Combined with horse blood

A

Chocolate Agar Plate

33
Q

Examples of Differential Media

A

Mac Conkey
Blood Agar Plate
Eosin methylene blue (EMB)
Hektoen enteric agar

34
Q

Media that differentiates lactose fermenters (pink colonies) from nonlactose fermenters (colorless colonies)

A

Mac Conkey

35
Q

Media that differentiates hemolytic pattern

A

Blood Agar Plate

36
Q

Examples of Selective media

A 
Hektoen enteric agar (HE)
MacConkey agar
Xylose lysine desoxycholate agar (XLD)
Bismuth sulfite agar (BSA)
Mannitol salt agar
Thayer Martin agar (TMA)
37
Q

Inhibitory substances for Gram-positive bacteria

A

Crystal/gentian violet
Basic/carbol fuschin
Bile salt

38
Q

Inhibitory substances for Gram-negative bacteria

A

Potassium tellurite

Sodium azide

39
Q

Inhibitory substances for Swarming bacteria

A

Alcohol

Chloral hydrate

40
Q

Media that is used to isolate bacteria with specific growth requirements

A

Special media

41
Q

Examples of Special media

A

Lowenstein Jensen medium (LJ)

Thiosulfate Citrate Bile Salt Sucrose agar (TCBS)

42
Q

Media that contains bile salt and dyes for nonenteric pathogens

A

Hektoen enteric agar (HE)

43
Q

Used to differentiate colonies of Salmonella (red with black centers) from Shigella (red or clear colonies without black centers)

A

Xylose desoxycholate agar (XLD)

44
Q

The most commonly performed inoculation technique

A

Streaking

45
Q

This technique is applied to a semi-solid medium

A

Stabbing

46
Q

It is used to inoculate organism into the slant and butt tube medium

A

Stab and streak technique

47
Q

Is a technique where the organisms is added to the molten agar, mixed and then allows the medium to solidify

A

Pour plate technique

48
Q

Are growth of microorganism on a culture medium

A

Cultures

49
Q

Is the doubling of cell number

A

Generation

50
Q

The time required for a bacterium to double its population

A

Generation time

51
Q

Period wherein there is no cell division

A

Lag Phase/ Period of Rejuvenescence

52
Q

Period wherein microorganisms are actively growing and dividing

A

Log/ Exponential Phase

53
Q

Period where in there is a balance between cell division and dying organisms

A

Stationary/ Plateau Phase

54
Q

Period wherein there is cessation of bacterial growth

A

Death/Decline phase

55
Q

Calculation of the final number of cells

A

Initial number of cells x 2n

Where in n = # of generation

56
Q

Most common asexual reproductive process whereby single cell divides into two daughter cells after developing a transverse cell wall

A

Transverse binary fission

57
Q

Growth can be measured by:

A

Cell count
Cell mass
Cell activity

58
Q

Methods for measuring bacterial growth

A 
Microscopic Count
Plate Count
Membrane or Molecular Filter
Turbidimetric Method
Dry Weight Determination
Biochemical Activity

Microbiology (4 decks)

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