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Culture and Culture Media Flashcards

1
Q

Is liquid, semi-solid or solid medium utilized to observe growth patterns or microorganism as well as for transport and storage

A

Culture Medium

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2
Q

Type of culture that is composed of only one specie

A

Pure culture

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3
Q

Type of culture that is composed of more than one species

A

Mixed culture

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4
Q

Culture that is composed of several species contained in a separate culture medium

A

Stock culture

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5
Q

Solidifying agent

A

Agar

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6
Q

A medium that contains 0% agar

A

Liquid medium

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7
Q

A semi-solid medium contains how much percent of agar? (range)

A

0.5-1%

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8
Q

A medium that contains 2-3% agar

A

Solid medium

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9
Q

Give examples of liquid medium

A

Brain Heart Infusion (BHI)
Trypticase Soy Broth (TSB)
Thioglycollate

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10
Q

Give an example of a semi-solid medium

A

Sulfide indole motility (SIM) medium

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11
Q

Give examples of a solid medium

A

Triple sugar iron (TSI) agar
Mac Conkey
Blood Agar Plate (BAP)
Chocolate agar plate (CAP)

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12
Q

What temperature will the agar melt (range)

A

80-90 degrees C

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13
Q

What temperature will the agar solidify (range)

A

40-50 degrees C

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14
Q

What is the cooling temperature for the distribution of agar into Petri plates (range)

A

55-60 degrees C

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15
Q

It is a medium where all the components are known to the user and it also used for research purposes

A

Synthetic or Defined medium

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16
Q

It is composed of some unknown substances such as peptones, meat and yeast extracts. It is very useful for isolation of bacteria.

A

Non-Synthetic or Complex medium

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17
Q

It is used for obligate intracellular bacteria

A

Tissue Culture medium

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18
Q

Examples of Non-Synthetic or Complex medium

A

Nutrient broth
Trypticase Soy Broth (TSB)
Mac Conkey agar

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19
Q

Examples of Tissue Culture Medium

A

W138
HELA 229 cells
Mc Coy cells

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20
Q

Classification of culture medium according to how it is dispensed/distributed

A

Plated medium

Tube medium

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21
Q

4 types of tube medium

A

Liquid
Slant
Butt and slant
Butt

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22
Q

Media that are routinely used in the laboratory and without added supplement. It supports the growth of most nonfastidious bacteria.

A

Simple media/ General Purpose media/ Supportive media

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23
Q

Media that is used to propagate the growth of certain group of bacteria from a mixture of organisms. It contains specific nutrients; liquid-type media.

A

Enrichment media

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24
Q

Media with added supplements such as blood, vitamins and yeast extracts necessary for the growth of fastidious organisms. These are solid-type media

A

Enriched media/ Nonselective media

25
Media that allow visualization of metabolic differences between groups of bacteria
Differential media
26
These are media incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the growth of the desired organisms
Selective media
27
Examples of Simple media
``` Nutrient Agar (NA) Nutrient Broth (NB) Trypticase Soy Broth (TSB) ```
28
Composition of Simple Media
Meat and soybean extracts
29
Examples of Enrichment media
``` Alkaline peptone water Selenite F Thioglycollate Tetrathionate Gram negative broth (GN) Lim broth ```
30
Examples of Enriched media
Blood Agar Plate (BAP) | Chocolate Agar Plate (CAP)
31
It is the standard nonselective culture medium
Blood Agar Plate
32
Used for the isolation of Hemophilus. Combined with horse blood
Chocolate Agar Plate
33
Examples of Differential Media
Mac Conkey Blood Agar Plate Eosin methylene blue (EMB) Hektoen enteric agar
34
Media that differentiates lactose fermenters (pink colonies) from nonlactose fermenters (colorless colonies)
Mac Conkey
35
Media that differentiates hemolytic pattern
Blood Agar Plate
36
Examples of Selective media
``` Hektoen enteric agar (HE) MacConkey agar Xylose lysine desoxycholate agar (XLD) Bismuth sulfite agar (BSA) Mannitol salt agar Thayer Martin agar (TMA) ```
37
Inhibitory substances for Gram-positive bacteria
Crystal/gentian violet Basic/carbol fuschin Bile salt
38
Inhibitory substances for Gram-negative bacteria
Potassium tellurite | Sodium azide
39
Inhibitory substances for Swarming bacteria
Alcohol | Chloral hydrate
40
Media that is used to isolate bacteria with specific growth requirements
Special media
41
Examples of Special media
Lowenstein Jensen medium (LJ) | Thiosulfate Citrate Bile Salt Sucrose agar (TCBS)
42
Media that contains bile salt and dyes for nonenteric pathogens
Hektoen enteric agar (HE)
43
Used to differentiate colonies of Salmonella (red with black centers) from Shigella (red or clear colonies without black centers)
Xylose desoxycholate agar (XLD)
44
The most commonly performed inoculation technique
Streaking
45
This technique is applied to a semi-solid medium
Stabbing
46
It is used to inoculate organism into the slant and butt tube medium
Stab and streak technique
47
Is a technique where the organisms is added to the molten agar, mixed and then allows the medium to solidify
Pour plate technique
48
Are growth of microorganism on a culture medium
Cultures
49
Is the doubling of cell number
Generation
50
The time required for a bacterium to double its population
Generation time
51
Period wherein there is no cell division
Lag Phase/ Period of Rejuvenescence
52
Period wherein microorganisms are actively growing and dividing
Log/ Exponential Phase
53
Period where in there is a balance between cell division and dying organisms
Stationary/ Plateau Phase
54
Period wherein there is cessation of bacterial growth
Death/Decline phase
55
Calculation of the final number of cells
Initial number of cells x 2n | Where in n = # of generation
56
Most common asexual reproductive process whereby single cell divides into two daughter cells after developing a transverse cell wall
Transverse binary fission
57
Growth can be measured by:
Cell count Cell mass Cell activity
58
Methods for measuring bacterial growth
``` Microscopic Count Plate Count Membrane or Molecular Filter Turbidimetric Method Dry Weight Determination Biochemical Activity ```

Microbiology (4 decks)

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