CSF PROTEIN DETERMINATION

Question 1

Preferred method for CSF Protein determination

Answer 1

Turbidimetric

Question 2

T/F.
Turbidimetric precipitates only albumin

Answer 2

FALSE.
Turbidimetric precipitates both albumin and globulin

Question 3

It precipitates albumin only:

Answer 3

sulfosalicylic acid

Question 4

To precipitate globulins, add

Answer 4

sodium sulfate

Question 5

Dye binding uses

Answer 5

Coomassie Brilliant Blue

Question 6

assess the integrity of the blood brain barrier

Answer 6

CSF /Serum Albumin Index

Question 7

CSF /Serum Albumin Index
Normal value:
Abnormal value:

Answer 7

Normal value: <9
Abnormal value: >9

Question 8

Index of 100:

Answer 8

complete damage to BBB

Question 9

assess condition with IgG production within the CNS (Multiple Sclerosis)

Answer 9

IgG Index

Question 10

indicative of IgG production within the CNS

Answer 10

IgG index

Question 11

IgG Index
Normal value:
Abnormal value:

Answer 11

Normal value: <0.70
Abnormal value: >0.70

Question 12

Done in conjunction with serum electrophoresis

Answer 12

CSF electrophoresis

Question 13

For the detection of oligoclonal bands

Answer 13

CSF electrophoresis

Question 14

The presence of 2 or more oligoclonal bands in CSF but not in serum is valuable for the diagnosis of:

Answer 14

Multiple sclerosis
Neoplastic disorders
Encephalitis
Guillain-Barre’ syndrome

Question 15

Protein component of the lipid-protein complex that insulate the nerve fibers

Answer 15

Myelin Basic Protein

Question 16

presence of MBP in CSF indicates destruction of

Answer 16

myelin sheath

Question 17

Used to monitor course of multiple sclerosis

Answer 17

Myelin Basic Protein

Question 18

____ techniques are used for measurement

Answer 18

Immunoassay

Question 19

Done in conjunction with blood glucose.

Answer 19

CSF Glucose

Question 20

Specimen for blood glucose should be drawn ____ to spinal tap

Answer 20

2 hours prior

Question 21

CSF Glucose Normal values

Answer 21

60-70% of the plasma glucose

Question 22

CSF Glucose is increased

Answer 22

Due to increase plasma glucose (not
significant)

Question 23

CSF Glucose is decreased in

Answer 23

Bacterial, Fungal, and Tubercular Meningitis

Question 24

CSF glucose is normal in

Answer 24

Viral Meningitis

Question 25

CSF lactate levels can be a valuable aid in diagnosing and managing ____

Answer 25

meningitis

Question 26

CSF lactate normal values

Answer 26

Normal value: 10-22 mg/dl (1.1 to 2.4 mmol/L)

Question 27

T/F. CSF Lactate is directly proportional to glucose

Answer 27

CSF lactate is inversely proportional to glucose

Question 28

Frequently CSF lactate levels are used to monitor patients with

Answer 28

severe head injuries

Question 29

RBCs contain high concentrations of lactate, and results that are falsely elevated may be obtained on

Answer 29

Xanthochromic or hemolyzed fluid

Question 30

CSF Lactate Values: Bacterial meningitis Fungal and tubercular meningitis Viral meningitis

Answer 30

Bacterial meningitis - Increased (>35mg/dl) Fungal and tubercular meningitis - Increased (>25mg/dl) Viral meningitis - Normal (remains lower than 25mg/dl)

Question 31

Product of ammonia and alpha-ketoglutarate

Answer 31

CSF Glutamine

Question 32

Indirect test for the presence of excess ammonia in CSF

Answer 32

CSF Glutamine

Question 33

Why CSF Glutamine is preferred over direct measurement of CSF ammonia?

Answer 33

because the glutamine concentration remains more stable than the volatile

Question 34

CSF Glutamine Normal value

Answer 34

8 to 18 mg/dl

Question 35

CSF Glutamine is increased in

Answer 35

Reye’s syndrome, Disturbance of consciousness/coma (more than 35 mg/dl)

Question 36

CSF Enzyme for Brain tissue LD

Answer 36

LD 1 and 2

Question 37

CSF Enzyme for Lymphocytes LD

Answer 37

LD 2 and 3

Question 38

CSF Enzyme for Neutrophils LD

Answer 38

LD 4 and 5

Question 39

Serum LDH Normal = Flipped pattern =

Answer 39

Normal = LDH 2>1>3>4>5 Flipped pattern = LD 1>2>3>4>5 (AMI)

Question 40

CSF LDH Normal = Neurological abnormalities= Bacterial meningitis=

Answer 40

Normal = LDH 2>3>1>4>5 Neurological abnormalities= LDH 1>2>3>4>5 Bacterial meningitis= LDH 5>4>3>2>1

Question 41

Detects gram-negative bacterial endotoxin

Answer 41

Limulus Lysate Test

Question 42

Limulus Lysate Test Reagent

Answer 42

Blood of horseshoe crab

Question 43

Positive result for Limulus Lysate Test

Answer 43

Clumping or clot formation