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PMCOL 200 Final > CRISPR > Flashcards

CRISPR Flashcards

1
Q

what is the function of ZFNs

a) directed evolution
b) clone new RVDs
c) order new guide RNA

A

a

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2
Q

what is the function of TALENs

a) directed evolution
b) clone new RVDs
c) order new guide RNA

A

b

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3
Q

what is the function of CRISPR/ Cas9

a) directed evolution
b) clone new RVDs
c) order new guide RNA

A

b

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4
Q

what year was CRISPR acronmym invented?

a) 2000
b) 2012
c) 2002
d) 2008

A

c 2002

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5
Q

what year was CRISPR acronmym invented?

a) 2000
b) 2012
c) 2002
d) 2008

A

c 2002

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6
Q

who got the nobel prize for for CRISPR gene editing applications

a) Banter and Brymes, 2005
b) Elliot and Aaron, 2012
c) Doudna and Charpentier, 2012
d) Saswati and Taylor, 2005

A

c

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7
Q

when was CRISPR sequence discovered

a) 1987, Hashimoto
b) 1990, Aaron
c) 1987, Ishino
d) 1990, Zuko

A

c

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8
Q

how is cas9 recruited to the DNA target site

a) mRNA: cRNA duplex
b) cRNA: taqMan assay duplex
c) tracrRNA: crRNA duplex
d) mRNA: tracrRNA duplex

A

c

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9
Q

a crRNA contains a _____ bp sequence _____ to the target (complementary/ antagonistic)

A

20, complementary

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10
Q

what acts as the bridge between the crRNA and the Cas9 enzyme

A

trans-activating cRNA (tracrRNA)

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11
Q

CRISPR is derived from

A

prokaryotic immune system

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12
Q

where does cas9 bind

A

guide RNA

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13
Q

what protein cleaves the invading DNA

A

cas9``

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14
Q

how is cas9 recruited to the target site?

A

cRNA/ tracrRNA duplex

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15
Q

what forms the bridge between cRNA and cas9

A

tracrRNA

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16
Q

HOW DOES cas9 cut DNA`

A

CAS9 ENDONUCLEASE

17
Q

WHAT CAN join together DNA fragments

A

NHEJ or HDR

18
Q

what does PAM stand for

A

protospacer associated motif

19
Q

to determine the 5’ start of the actual sgRNA sequence count ____ bp _____(upstream or downstream )of the PAM

A

20 bp , upstreak

20
Q

what is cas associated with in base editing

A

deaminase

21
Q

in prime editing what is cas9 associated with

A

reverse transcriptase

22
Q

what kind of cas9 if used in CRISPRa/i

A

dcas9

23
Q

what is dcas9 fused to in CRISPRa

A

transcriptor activator

24
Q

what is dcas9 fused to in CRISPRi

A

transcription repressor

25
Q

what can be treated in vivo with CRISPR

A

ear and eye

26
Q

what can ex vivo therapy be used for

A

blood sickle cell diseases

27
Q

what kind of cas9 is used for epigenome editing

A

dcas9-p300

28
Q

what kind of cas9 is used in prime editing

A

PMCOL 200 Final (11 decks)

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