CRISPR Flashcards
what is the function of ZFNs
a) directed evolution
b) clone new RVDs
c) order new guide RNA
a
what is the function of TALENs
a) directed evolution
b) clone new RVDs
c) order new guide RNA
b
what is the function of CRISPR/ Cas9
a) directed evolution
b) clone new RVDs
c) order new guide RNA
b
what year was CRISPR acronmym invented?
a) 2000
b) 2012
c) 2002
d) 2008
c 2002
what year was CRISPR acronmym invented?
a) 2000
b) 2012
c) 2002
d) 2008
c 2002
who got the nobel prize for for CRISPR gene editing applications
a) Banter and Brymes, 2005
b) Elliot and Aaron, 2012
c) Doudna and Charpentier, 2012
d) Saswati and Taylor, 2005
c
when was CRISPR sequence discovered
a) 1987, Hashimoto
b) 1990, Aaron
c) 1987, Ishino
d) 1990, Zuko
c
how is cas9 recruited to the DNA target site
a) mRNA: cRNA duplex
b) cRNA: taqMan assay duplex
c) tracrRNA: crRNA duplex
d) mRNA: tracrRNA duplex
c
a crRNA contains a _____ bp sequence _____ to the target (complementary/ antagonistic)
20, complementary
what acts as the bridge between the crRNA and the Cas9 enzyme
trans-activating cRNA (tracrRNA)
CRISPR is derived from
prokaryotic immune system
where does cas9 bind
guide RNA
what protein cleaves the invading DNA
cas9``
how is cas9 recruited to the target site?
cRNA/ tracrRNA duplex
what forms the bridge between cRNA and cas9
tracrRNA
HOW DOES cas9 cut DNA`
CAS9 ENDONUCLEASE
WHAT CAN join together DNA fragments
NHEJ or HDR
what does PAM stand for
protospacer associated motif
to determine the 5’ start of the actual sgRNA sequence count ____ bp _____(upstream or downstream )of the PAM
20 bp , upstreak
what is cas associated with in base editing
deaminase
in prime editing what is cas9 associated with
reverse transcriptase
what kind of cas9 if used in CRISPRa/i
dcas9
what is dcas9 fused to in CRISPRa
transcriptor activator
what is dcas9 fused to in CRISPRi
transcription repressor
what can be treated in vivo with CRISPR
ear and eye
what can ex vivo therapy be used for
blood sickle cell diseases
what kind of cas9 is used for epigenome editing
dcas9-p300
what kind of cas9 is used in prime editing
