Creatine Kinase Flashcards
What is the pH of the buffer used in the experiment
Imidazole buffer (6.7 pH)
Optimal ph of Oliver rosalki method
6.3 pH
What is the purpose of N-acetylcysteine?
Source of sulfhydryl compounds to partially reverse the inactivation of CK caused by the oxidation of sulfhydryl groups
Does the absorbance in CK increases or decreases
Increase in absorbance
What enzyme is Hexokinase?
Transferase
What enzyme is G6PD?
Oxidoreductase
What is the antibody used in the CK experiment?
Anti-M from goat
Why is the enzyme in CK-MB from goat and not humans?
Because goat CK is foreign, therefore antibodies will be formed. Aside from this, since enzymes are proteins, they are highly immunogenic.
What does anti M inhibit? How much for each CKMM CKMB CKBB?
CKMM - 100%,
CKMB - 50%
CKBB - none
