Conventionally Cultured Bacteria Flashcards
List 3 Major distinguishing features of Staphylococcus
- Gram +ve
- Cocci in clusters
- Catalase +ve
What were the two Gram +ve aerobic cocci groups covered in class?
Staphylococcus and streptococcus
What two Staphylococci species were presented?
- S. aureus
2. Coagulase negative Staphylococci (CNS)
What are two virulence factors of S. aureus?
- Toxic Shock Syndrome Toxin 1 (TSST-1)
2. Enterotoxins
What 7 clinical presentations is S. aureus associated with?
- SSTI
- Necrotizing fasciitis
- Osteomyelitis
- Endocarditis
- Toxic shock syndrome
- Cystic fibrosis pneumonia
- Diarrhea
Primary pathogenicity of CNS, such as S. epidermidis?
Biofilms
Clinical significance of CNS (2)
- Nosocomial bloodstream infections from IV catheters
2. Prosthetic material infections
3 major distinguishing features of Streptococcus species
- Gram +ve
- Cocci in pairs and chains
- Catalase -ve
List all Streptococcus species presented in class (5)
- Group A Streptococcus (S. pyogenes)
- Group B Streptococcus (S. agalactiae)
- S. pneumoniae
- S. mitis
- Enterococci
What are the 2 B-hemolytic Strep species presented?
- GAS (S. pyogenes)
2. GBS (S. agalactiae)
What are the 2 a-hemolytic strep species that were presented?
- S. pneumoniae
2. S. mitis
What was the only non-hemolytic strep species presented?
Enterococci
Pathogenicity of GAS
Streptolysin O and S
Pathogenicity of GBS
Polysaccharide capsule
Pathogenicity of S. pneumoniae
Polysaccharide capsule
Pathogenicity of S. mitis
Polysaccharide capsule
Pathogenicity of enterococci
Surface adhesins
Diseases associated with GAS (4)
- Pharyngitis
- Necrotizing fasciitis
- Rheumatic fever
- Streptococcal Toxic Shock Syndrome (STSS)
Diseases associated with GBS (2)
- Early onset neonatal disease (pneumonia, sepsis)
2. Late onset neonatal disease (meningitis, sepsis)
Diseases associated with S. pneumoniae (4)
- Community acquired pneumonia
- Otitis media
- Conjunctivitis
- Meningitis
Diseases associated with S. mitis (2)
- Endocarditis
2. Bacteremia
Diseases associated with enterococci (3)
- UTI
- Endocarditis
- Nosocomial VRE
Aerobic Gram +ve bacilli groups presented (2)
- Corynebacterium
2. Listeria
Major features of Corynebacterium (4)
- Gram +ve
- Rods
- Non-branching
- Catalase +ve
2 examples of Corynebacterium
- C. diphtheriae
2. C. jeikeium
Pathogenicity and disease associated with C. diphtheriae
- Diphtheria toxin
2. Diphtheria
C. jeikeium has unknown pathogenicity, but causes this type of infection(s)
Foreign body infections
5 features of Listeria
- Gram +ve
- Single or short chain rods
- Non-branching
- Motile
- Catalase +ve
Pathogenicity of L. monocytogenes (1)
- Hemolysin
6 problems caused by L. monocytogenes
- Gastroenteritis
- Meningitis
- Encephalitis
- Abortion
- Stillbirth
- Preterm labour
Anaerobic Gram +ve cocci genus presented (1)
Peptostreptococcus
3 features of Peptostreptococcus
- Elongated cocci
- Gram +ve
- Non-spore forming
Pathogenicity of Peptostreptococcus is unknown, what is its clinical significance? (4)
- Cutaneous
- Respiratory
- Oral
- Female pelvic infections
Often mixed infections
Anaerobic Gram +ve bacilli (2 genus)
- Actinomyces
2. Clostridium
2 features of Actinomyces
- Gram +ve
2. Branching, filamentous bacilli
Actinomyces israelii has unknown pathogenicity and causes 2 things
- Cervicofacial infections
2. Pelvic infections (IUD)
5 features of Clostridium spp.
- Gram +ve
- Box car rods = C. perfringens
- Straight rods = C. difficile, C. tetani
- Straight/curved rods = C. botulinum
- Spores
4 Clostridium species
- C. tetani
- C. difficile
- C. perfringens
- C. botulinum
2 toxins made by C. perfringens
- a - toxin
2. Enterotoxin
3 diseases associated with C. perfringens
- Gas gangrene
- Bloody diarrhea
- Acute necrotizing GI infection
2 toxins and disease caused by C. difficile
- Toxin A
- Toxin B
- Diarrhea
2 genus of aerobic Gram -ve fermenters mentioned in class
Enterobacteriaceae
Vibrio spp.
The group Enterobacteriaceae includes the following 4 organisms
- E. coli
- Enterobacter
- Salmonella
- Proteus
3 features of Enterobacteriaceae
- Gram -ve
- Rods
- Oxidase -ve
Virulence factors of E. coli (4)
- Endotoxin
- Exotoxins
- Capsule
- Pili
3 infections caused by E. coli
- Meningitis in newborns
- UTI
- GI infection
3 pathogenic factors of Enterobacter spp.
- Exotoxins
- Endotoxins
- Capsules
3 diseases associated with Enterobacter spp.
- UTI
- Nosocomial infections
- Blood infections
Pathogenicity of Salmonella spp. (3)
- Adhesion proteins
- Survival in phagocytes
- Dissemination to other tissues
2 common infections associated with Salmonella spp.
- GI infections
2. Bacteremia
Pathogenicity of Proteus spp. (4)
- Exotoxins
- Endotoxins
- Capsules
- Adhesion proteins
3 infections associated with Proteus spp.
- UTI
- Nosocomial infections
- Blood infections
3 major features of Vibrio
- Gram -ve
- Rods
- Oxidase +ve
Pathogenicity of V. cholerae (2)
- Somatic O antigens
2. Exotoxins
Aerobic Gram -ve non-fermenters shown in class include these 2 groups
- Moraxella
2. Pseudomonas
3 major features of Moraxella
- Gram -ve
- Diplococci
- Oxidase +ve
Moraxella catarrhalis has which pathogenicity and causes what disease…
- Uncertain, but has cell envelope adhesion factors
2. Community acquired pneumonia
Major features of Pseudomonas (5)
- Gram -ve
- Slender bacilli
- Oxidase +ve
- Metallic sheen
- Grape-like odor
Pseudomonas aeruginosa has exotoxins and endotoxin - what infections is it associated with? (4)
- Otitis externa
- Pneumonia in cystic fibrosis
- Ventilator acquired pneumonia
- UTI
Haemophilus and Neisseria belong to which category of pathogen we learned about?
Fastidious Gram -ve bacilli/cocci/coccobacilli
4 features of Haemophilus spp.
- Gram -ve
- Coccobacilli or short rods
- Satellite
- Oxidase +ve
2 virulence factors of Haemophilus influenzae
- Capsule
2. Outer membrane protein
5 clinical presentations of H. influenzae infection
- Meningitis
- Epiglottitis
- Conjunctivitis
- Otitis media
- Pneumonia
3 features of Neisseria spp.
- Gram -ve
- Diplococci
- Oxidase +ve
Genus Neisseria includes these 2 species
- N. meningitidis
2. N. gonorrhea
Pathogenicity of N. meningitidis (2)
- Capsule
2. Outer membrane protein
Pathogenicity of N. gonorrhea (4)
- Pili
- Capsule
- Proteases
- Adherence proteins
3 clinical presentations of N. gonorrhea
- Gonorrhea
- Pharyngitis
- Conjunctivitis
Bacteroides is a “BLANK” Gram “BLANK”
Anaerobic, negative
Pathogenicity of Bacteroides fragilis (3)
- Capsule
- Endotoxin
- Enzymes
Clinical significance of Bacteroides fragilis (3)
- Abscesses throughout body
- Bacteremia
- Aspiration pneumonia
“BLANK” is the only genus of aerobic gram -ve fermenters shown in class
Vibrio