Colorimetry Flashcards
what is colour/how is it seen
depending on something chemical composition, different materials will absorb and reflect light of different wave lengths
the colour we observe…
is the colour reflected and is complementary colour of the most strongly absorbed wavelength
what is colorimetry
a technique used to measure the concentration of ions by the amount of light that is able to pass through a solution
how is colorimetry a qualitative technque
because it is used to dtermine the indentity of the solute in a solution
how is colorimetry a quantitative technque
because it is used to determine the concentration of a solute in a solution
what are the steps in colorimetry
- light from a light source is ttransmitted through a filter to select the wavelength of light most readily absorbed by the solute 2. solute ions in the test tuube absorb a particular amount of light 3. an electronic detector is used to measure the percentage of light absorbed by the solution
how is a calibration curve of absorbance vs concentration created
through measuring the different absorbances of standard soolutions of known concentration
monochromator
like colorimetry but more accurate and can select for a single wavelength of light ti use
steps of UV spectroscopy/monochromator
the sample is first scanned across multiple wavelenth to assess which is most absorbed by the solution, then we use the strongest absorbed wavelengt6h
applications of colorimetryy and uv spectroscopy
identify the contents ioiif blood, assessing the contents for toxicity markers and testing the air for the presence of chromium and lead