Colorimetry Flashcards

1
Q

what is colour/how is it seen

A

depending on something chemical composition, different materials will absorb and reflect light of different wave lengths

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2
Q

the colour we observe…

A

is the colour reflected and is complementary colour of the most strongly absorbed wavelength

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3
Q

what is colorimetry

A

a technique used to measure the concentration of ions by the amount of light that is able to pass through a solution

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4
Q

how is colorimetry a qualitative technque

A

because it is used to dtermine the indentity of the solute in a solution

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5
Q

how is colorimetry a quantitative technque

A

because it is used to determine the concentration of a solute in a solution

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6
Q

what are the steps in colorimetry

A
  1. light from a light source is ttransmitted through a filter to select the wavelength of light most readily absorbed by the solute 2. solute ions in the test tuube absorb a particular amount of light 3. an electronic detector is used to measure the percentage of light absorbed by the solution
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7
Q

how is a calibration curve of absorbance vs concentration created

A

through measuring the different absorbances of standard soolutions of known concentration

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8
Q

monochromator

A

like colorimetry but more accurate and can select for a single wavelength of light ti use

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9
Q

steps of UV spectroscopy/monochromator

A

the sample is first scanned across multiple wavelenth to assess which is most absorbed by the solution, then we use the strongest absorbed wavelengt6h

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10
Q

applications of colorimetryy and uv spectroscopy

A

identify the contents ioiif blood, assessing the contents for toxicity markers and testing the air for the presence of chromium and lead

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