Collagen

Question 1

What are the stages that make collagen?

Answer 1

ER: prepro alpha chain –> pro alpha chain + OH (require prolyl hydroxylase that needs Vit C) for H bonds + disulphides bonds = triple helix + N-C extension to stop large unit forming
GOLGI: + glucose, exocytosis = procollagen
EXTRACELLULAR: peptidases remove N-C extensions on procollagen = tropocollagen + covalent cross links (requires Vit B)= collagen

Question 2

What is collagens structure?

Answer 2

3 α chains –> tropocollagen (glycine every 3rd), H bonds

Question 3

Why is tropocollagen secreted outside of the cell?

Answer 3

Tropocollagen forms collagen, don’t want to make inside the cell, too big = cell lysis

Question 4

What cell type secrets collagen?

Answer 4

Fibroblasts

Question 5

What is the most abundant protein in the body?

Answer 5

Collagen

Question 6

What’s is collagens structural properties?

Answer 6

Non-extendable, non-compressible, high tensile strength

Question 7

What would a mutation in collagen result in?

Answer 7

Mutation = incorrect folding of collagen as the correct bonds cannot form = lower strength

Question 8

What bonds are present in collagen?

Answer 8

H bonds, covalent bonds cross-linking

Question 9

Where does collagen synthesis take place?

Answer 9

ER, Golgi, extracellular space

Question 10

What is collagens AA sequence?

Answer 10

(X-Y-Gly)n X/Y: proline or hydroproline. Gly in every 3rd position