CNS Development Flashcards

1
Q

What are the three layers of the early neural tube?

A

Ectoderm

Mesoderm

Endoderm

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2
Q

Describe the early development of the neural tube and state when this occurs.

A
  • At 3 weeks
  • Proliferation of the ectoderm –> neural plate
  • Neural plate thickens and folds up on the sides to fuse dorsally –> neural canal is formed in the space
  • A little strip of cells at the dorsal tip of the neural fold forms the neural crests that run alongside the neural canal more dorsally.

Neural tube –> ALL CNS cells

Neural crest –> ALL PNS cells

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3
Q

What is the wall of the neural tube made of? What deoes it give rise to?

A

Neuroepithelium –> All cells of the CNS

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4
Q

What 3 types of cells differentiate from the neuroepithelium?

A

Neuroblasts -All neurons with cell bodies in the CNS

Glioblasts - Astrocytes, oligodendrocytes (neuroglia, NB microglia arise elsewhere)

Ependymal cells - Lining ventricles and central canal

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5
Q

What do neural crest cells differentiate into?

A

Sensory neurones of dorsal root ganglia and cranial ganglia.

Postganglionic autonomic neurones.

Schwann cellsmyelinate axons in PNS.

Non-neuronal derivativese.g. melanocytes.

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6
Q

Describe the proliferation of the neuroepithelium.

A
  • Cross-section of the neural tube at an early stage.
  • There is ONE-layer of cells and most cells are attached to both the inner and outer membrane.
  • The bigger cells towards the inside are undergoing mitosis.
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7
Q

Describe the differentiation of the neuroepithelium.

A
  • Mitosis occurs at the inner side after moving downward from outside layer
  • One of daughter cells migrates and develops into neuroblasts while the other stays attached, goes into the cell cycle again and gets bigger.
  • They develop processes to direct them away from inner membrane and you end up with three layers which starts to form difference between white and grey matter:
  1. Mainly mitosis occurring
  2. Mainly cell bodies (grey)
  3. Mainly axons (white)

NB: Glioblasts can migrate like this to white matter too but do not develop axons (processes instead)- they can migrate to grey matter too. Oligodendrocytes mainly in white. Ependymal cells remain in ependymal layer.

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8
Q

What are the layers of the neural tube?

A

There are 3 clear layers:

  • Inner – Ependymal layergerminal layer.
  • Middle – Grey matter – mantle layer.
  • Outer – White matter – marginal layer.
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9
Q

What controls differentiation of the neural tube?

A
  • Signalling molecules, secreted by surrounding tissues, interact with receptors on neuroblasts
  • These control migration & axonal growth by attraction and repulsion
  • Depends on concentration gradient & timing
    • Concentration gradient – close to source of molecules = higher conc.
    • Timing – only produce s. molecules when developing neurones have the correct receptors to receive them.
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10
Q

What 2 layers is the grey matter split into in the developing spinal cord ?

A

Alar plate - dorsally - made of interneurons - receive sensory info

Basal plate - ventrallly - motor neurons and interneurons - can send information out of the ventral roots

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11
Q

Describe three changes that occur in spinal cord development to form the matrue spinal cord.

A

Dorsally, alar (roof) plate –> eventually form dorsal horns

Ventrally, basal (floor) plate–> ventral horns

Laterally, neural crest cells –> sensory neurones of PNS in dorsal root ganglia

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12
Q

Describe flow of information in the mature spinal cord.

A

Dorsal root –> dorsal horn –> up to CNS (or reflex) –> ventral root –> motor neurone out

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13
Q

How does the brainstem develop from the spinal cord?

A
  • The development of the 4th ventricle occurs:
  • The roof plate proliferates rapidly and the dorsal part expands laterally so that the alar (roof) plate splits
    • Dorsal/sensory nuclei = lateral
    • Efferent/motor nuclei = medial
    • Nuclei are now arranges in columns based on function
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14
Q

Describe the structure and development of the brain in the embryo at about 5 weeks.

A
  • Only the anterior portion of the neural tube develops into the brain.
  • Three primary vesicles form:
    • Prosencephalonfuture forebrain.
    • Mesencephalonfuture midbrain.
    • Rhombencephalonfuture hindbrain.
  • Flexures formed: cephalic, pontine, cervical. These become more exaggerated upon development.

Over the next week, the most superior vesicle divides in two and the third (inferior) vesicle divides into two. This produces 5 secondary vesicles.

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15
Q

What are the 3 main flexures of the brain at development?

A

Flexures formed: cephalic, pontine, cervical.

These become more exaggerated upon development.

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16
Q

Describe the development of the brain at approx. 5 weeks.

A
  1. Forebrain develops into telencephalon (mostly cortex) + diencephalon (key as relay station for pathways -thalamus, hypothalamys, epithalamus and subthalamus)
  2. Hindbrain shows differentiation between pons + medulla
17
Q

Describe the development of the brain at approx. 8 weeks.

A
  • Hemisphere develops
  • Cerebellum development begins - from back of pons
  • Ventricular system develops - space within the neural tube becomes smaller relative to the wall forming centricular system
18
Q

What are the zones of the developing cortex?

A
  1. Marginal
  2. Cortical plate
  3. Intermediate
  4. Subventricular
  5. Ventricular
19
Q

Describe the development of the cortex.

A

NB: the grey matter is outside and white matter underneath in the brain (opposite for the spinal cord)

  1. Brain has a 6 layer structure (cerebellum - 3)
  2. Neurons develop from ventricular zone, follow processes of radial glial cells (act as a scaffold) and migrate to the marginal zone
  3. Radial glial cells have soma attached to inner membrane and send a single long process to the outer membrane
  4. Proliferation and migration occurs in waves until 6 layers have developed
20
Q

Which cells act as a scaffold in cortical development?

A

Radial glial cells

21
Q

List the processes which need to be coordinated in brain and spinal cord development to prevent disorders occurring.

A
  1. Proliferation
  2. Migration
  3. Differnetiation
  4. Axon growth
  5. Synapse formation
22
Q

Describe these neural tube defects:

  • Craniorachischisis
  • Anencephaly
  • Enceephalocele
  • Iniencephaly
A
  1. Craniorachischisis - completely open brain and spinal cord
  2. Anencephaly - open brain and lack of skull vault
  3. Encephalocele - herniation of the meninges and brain
  4. Iniencephaly - occipital skull and spine defects with extreme retroflexion of the head
23
Q

Describe these neural tube defects:

  • Spina bifida occulta
  • Closed spinal dysraphism
  • Meningocele
  • Myelomeningocele
A
  • Spina bifida occulta (deficiency of folic acid) - closed asymptomatic NTD in which some of the vertebrae are not completely closed
  • Closed spinal dysraphism - deficiency of at least two vertebral arches (on picture below covered by a lipoma)
  • Meningocele - protrusion of the meninges (filled with CSF) through a defect in the skull or spine
  • Myelomeningocele - open spinal cord (with a meningeal cyst)