Cloning

Question 1

In therapeutic cloning what 2 donations are required

Answer 1

An egg cell (with nucleus removed) and nucleus from a cell of the patient

Question 2

In therapeutic cloning the modified egg cell is stimulated to allow it to ____________

Answer 2

Divide

Question 3

In therapeutic cloning how long are the stem cells left for before being taken and used on the patient

Answer 3

4-5 days

Question 4

What animal is used to inject the antigen into during the production of monoclonal antibodies

Answer 4

Mouse

Question 5

What does the mouse produce when injected with the antigen and what does it do

Answer 5

B-Lymphocytes - They produce specific antibodies to the specific antigen injected

Question 6

What cells are collected from the mouse during the production of monoclonal antibodies

Answer 6

Spleen cells

Question 7

During the production of monoclonal antibodies what are the B-lymphocytes of the mouse fused with and what do you call the new cell produced

Answer 7

A myeloma cell (cancerous white blood cell) is fused with the mouse spleen to form a Hybridoma cell

Question 8

What characteristics of a Hybridoma cell make it effective at producing monoclonal antibodies

Answer 8

They divide rapidly while producing more antibodies specific to an antigen