clinical dec lab data

Question 2

IMMUNOASSAYS1.• Can detect picomolar concentrations 2. Can distinguish between T3 and T4 and between different steroids- high specificity of ___recognition3. Very precise4. High sample throughputs5. Cheap

Answer 2

antigen

Question 3

SANDWICH ASSAY1 The mAb + Hormone are mixed: 2 A second, radiolabelled Ab 3 The ‘sandwich’ is large enough to ppt. out when cooled, and then separated by ____4 Can then measure radioactivity, and plot this against the known amount of hormone which was added–> 5 Do this for about 8 different concs of hormone, then plot ‘calibration curve’ 6 At the same time as known hormone is added, patient’s plasma is also added in a separate tube of Ab7 This plasma will have been __diluted.repeat step 4+5• The ‘sandwich assay’ only works for hormones with a high molecular weight

Answer 3

centrifugationserial

Question 4

COMPETETIVE BINDING ASSAY• Used to measure concs of hormones with small molecular mass (Mw

Answer 4

Thyroid hormones and All steroid hormonesELISA:• Works on same principles except that the label attached to the 2nd Ab is an enzyme, which generates colour when a substrate is added