CIULLA_AEROBIC GRAM POSITIVE BACTERIA

Question 1

Aerobic gram-positive bacteria

Answer 1

Staphylococcus spp.
Micrococcus
Streptococcus
Enterococcus
Gemella
Leuconostoc
Abiotrophia
Granulicatella
Listeria monocytogenes
Corynebacterium
Arcanobacterium
Erysipelothrix rhusiopathiae
Nocardia asteroides
Bacillus

Question 2

What percent of the population carries S. aureus

Answer 2

30% of the population carries S.aureus as resident flora

Question 3

For people carrying S.aureus, the bacteria resides primarily in _________

Answer 3

anterior nares

Question 4

S. aureus is isolated from ________.

Answer 4

abscesses
wound infections
carbuncles

Question 5

S. aureus causes food poisoning via what component?

Answer 5

enterotoxin

Question 6

S. aureus is capable of causing _______.

Answer 6

food poisoning (via enterotoxin), pneumonia, osteomyelitis, endocarditis, wounds, staphylococcal scalded skin syndrome (SSS)

Question 7

How many types of enterotoxin does S. aureus produce?

Answer 7

6

along with toxic shock syndrome-1 (TSST-1)

Question 8

S. aureus arrangement

Answer 8

cluster

Question 9

S. aureus colonies appearance

Answer 9

opaque, smooth

Question 10

S. aureus hemolytic pattern in SBA

Answer 10

beta hemolytic

S. aureus also grows well in most media

Question 11

S. aureus characteristic

Catalase ( )
Coagulase ( )

Answer 11

Catalase (+)

Coagulase (+)

Question 12

Latex agglutination assay detects ________ on surface of S. aureus

Answer 12

clumping factor and protein A

Question 13

S. aureus

(+/-) ability to metabolize substrate pyrrolidonyl-a-naphthylamide

(+/-) ability to metabolize substrate ornithine

Answer 13

S. aureus

(-) PYR
(-) ornithine

Question 14

S. aureus appears _______ on MSA

Answer 14

yellow

S. aureus is capable of fermenting mannitol and therefore produces yellow colonies. Most coagulase-negative staphylococci do not ferment mannitol and therefor produce red colonies

Question 15

Penicillin-resistance of S.aureus is due to production of _________.

Answer 15

beta-lactamase

Methicillin-resistant S. aureus is resistant to B-lactam antibiotics because of production of altered penicillin-binding proteins.

Question 16

VISA

Answer 16

Vancomycin-intermediate S. aureus

rare

Question 17

VRSA

Answer 17

Vancomycin-resistant S. aureus

rare

Question 18

Vancomycin resistance of S. aureus is due to

Answer 18

VanA operon

This alters the target of vancomycin in the cell wall.
VISA occurs following overproduction of the target

Question 19

T/F:
Coagulase-negative Staphylococci are very common skin flora and are
mostly nonpathogenic

Answer 19

TRUE

They can however, cause disease in immunosuppressed and neutropenic patients.

Question 20

Coagulase-negative Staphylococci is associated with what infections

Answer 20

UTI and infections with catheters and shunts

Question 21

Arrangement of coagulase-negative Staphylococci

Answer 21

in clusters

Question 22

Coagulase-negative staphylococci color on blood agar

Answer 22

white to gray

Question 23

Hemolytic pattern of coagulase-negative staphylococci

Answer 23

nonhemolytic

Question 24

Coagulase-negative staphylococci characteristics

Catalase ( )
Coagulase ( )

Answer 24

Coagulase-negative ( ) staphylococci

Catalase (+)
Coagulase (-)