CIRCO

Question 1

Function of ORF 1 in Circo

Answer 1

ORF 1 = replicase protien

Question 2

Function of ORF-2 in Circo

Answer 2

ORF 2 = capsid protein

Question 3

Give (3) major ORF of Gyrovirus oriented in same direction from complementary (+) strand

Answer 3

ORF 1 = capsid protein
ORF 2 = protein phosphatase
ORF 3 = apoptin*

Question 4

Genome organization of Porcine circovirus 1
(only 2)

Answer 4

cap gene = capsid protein, expressed from spliced transcript

rep gene = directs the synthesis of 2 distinct proteins using differentially-spliced transcripts

Question 5

Genome organization of Chicken Anemia Virus (3)

Answer 5

ORF 1 (cap gene) = encodes the capsid protein VP1

VP2 = protein phosphatase, encoded by ORF 2

VP3 = apoptin, responsible for cell lysis, encoded by ORF 3

Question 6

Natural route of entry of chicken anemia virus

Answer 6

Ingestion of feces

Question 7

Clinical signs of PBFDV

Answer 7

• permanent loss of feathers
• beak & claw deformities
• botryoid = basophilic intracytoplasmic inclusions

Question 8

PDNS affects (2) organs and what type of hypersensitivity?

Answer 8

skin & LN
Type III hypersensitivity

Question 9

Polymerase circo uses in S-phase?

Answer 9

DdDp

Question 10

How is circo released from cell?

Answer 10

Cell lysis