Chromotography and Electrophoresis Flashcards
1
Q
What is chromatography?
A
The separation of small molecules based on their mass and solubility in solvent
2
Q
What increases the distance a substance moves up the chromatography paper?
A
If a substance is more soluble and the smaller it is
3
Q
How do we calculate the retention value?
A
Distance the spot has travelled/ Distance from the line to the solvent front
4
Q
What do you do if 2 pigments have similar solubility?
A
- You can use 2 way chromatography
- You turn the chromatogram on its side and you use different solvents to separate the pigments
5
Q
What is electrophoresis?
A
It uses electric currents to separate out amino acids and also larger proteins and DNA
6
Q
What is the charge density?
A
Charge and mass
