chromatography Flashcards
1
Q
TLC
A
Thin layer chromatography
2
Q
Why is paper used in TLC?
A
paper is made of cellulose- which has water molecules which are bonded to cellulose during the manufacturing process
3
Q
why is pencil used in paper chrom?
A
it is insoluble so will not interrupt investigation
4
Q
How can compounds be not colourless?
A
conjugated- having alternating single and double/triple bonds
5
Q
How can colourless compounds be identified?
A
By spraying with ninhydrin or UV
6
Q
limitations of TLC
A
similar compounds have similar Rf values
unknown compounds have no reference retention time
a small peak could hide under a larger peak
7
Q
limitations of gas chromatography
A
similar compounds have similar retention time
unknown compounds have no reference time
a small peak could hide under a larger peak
