Chromatography Flashcards
what is chromatography
a tenchnique which can be used to separate the components of mixtures
explain the how the 2 phases work
the phase flows through the stationary phase and carries the components of the mixture with it
what does chromatography test
the difference in the abilities of components to dissolve in the mobile phase and the difference in abilities of components to stick fast to the solid SP
how does this relate to IMFs
the more strongly a substance sticks to SP, the slow it moves in the direction of flow of MP this relates to the strength of IMF of attraction between solute and solvent
for paper chrome was is the SP, MP and mixture
SP - paper
MP - water (solvent)
mixture - texta (solute)
for gas chrome was is the SP, MP and mixture
SP - porous solide
MP - gas
Mixture - eg blood
explain gas chrome and what results it gives
the time the mixture takes to travel through tube will depend on how much time is spent with gas rather than attached to liquid
explain retention time
a component should always travel at the same speed, it’s identity can be found by comparing the distance it moves relative to the solvent
what does retention time depend on
higher boiling point and greater solubility - long retention time