Chromatography Flashcards

1
Q

what is chromatography

A

a tenchnique which can be used to separate the components of mixtures

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2
Q

explain the how the 2 phases work

A

the phase flows through the stationary phase and carries the components of the mixture with it

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3
Q

what does chromatography test

A

the difference in the abilities of components to dissolve in the mobile phase and the difference in abilities of components to stick fast to the solid SP

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4
Q

how does this relate to IMFs

A

the more strongly a substance sticks to SP, the slow it moves in the direction of flow of MP this relates to the strength of IMF of attraction between solute and solvent

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5
Q

for paper chrome was is the SP, MP and mixture

A

SP - paper
MP - water (solvent)
mixture - texta (solute)

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6
Q

for gas chrome was is the SP, MP and mixture

A

SP - porous solide
MP - gas
Mixture - eg blood

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7
Q

explain gas chrome and what results it gives

A

the time the mixture takes to travel through tube will depend on how much time is spent with gas rather than attached to liquid

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8
Q

explain retention time

A

a component should always travel at the same speed, it’s identity can be found by comparing the distance it moves relative to the solvent

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9
Q

what does retention time depend on

A

higher boiling point and greater solubility - long retention time

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