Chromatography

Question 1

What are the types of chromatography?

Answer 1

Paper chromatography
Thin layer chromatography (TLC)
Gas chromatography (GC)
High-performance Liquid Chromatography (HPLC)

Question 2

Retardation factor is what?

Answer 2

A measure if distance that component travelled, divided by the distance the solvent front travelled.

Question 3

What is retention time?

Answer 3

Time taken for a component to emerge from the column in either a gas chromatograph or a high performance liquid chromatograph

Question 4

What is the mobile phase of chromatography?

Answer 4

It may be a gas or a solvent mixture, the mobile phase moves past the stationary phase

Question 5

What is a stationary phase in chromatography?

Answer 5

All times of chromatography have a stationary phase. In paper chromatography it is the paper. In thin layer chromatography it is the fine grace of alumina or silica gel. In gas chromatography it is either a solid or a liquid. In the high performance liquid chromatography it is a solid.

Question 6

Adsorption definition

Answer 6

The temporary binding of a component to the stationary phase in chromatography.

Question 7

Desorption definition

Answer 7

The movement into the mobile phase by a component during chromatography.

Question 8

Absorption definition

Answer 8

The intensity of light remaining after some has been absorbed compared with the intensity of light when none as been absorbed.

Question 9

What is the difference between absorption and adsorption?

Answer 9

Absorption is all about light and the emission spectrum whereas adsorption is about substances and chromatography.