Chromatography Flashcards
Name 3 types of chromatography
Thin layer
Column
Gas
How does TLC work
plate coated in solid such as silica and solvent moves up the plate
How does CC work
column is packed with a solid
solvent moves down the column
How does GC work
column packed with solid/solid coated with liquid
gas passed through the column at high temperature
How does separation in chromatography work
Depends on the balance between solubility of the moving phase and retention by the stationary phase
What can be used to analyse components separated by GC
mass spectrometry
Explain why it may be necessary to use more than one solvent in TLC
some components may not be soluble in both solvents
some components may hve the same rf value in one of the solvents