Chromatography Flashcards

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1
Q

What is chromatography used for?

A

-used to separate a mixture into it’s individual components
-relies on different solubilities of different chemicals in a mixture

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2
Q

How does chromatography work?

A

-components separate as the mobile phase travels over the stationary phase
-higher solubility = travels further up

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3
Q

Method of putting mixture on paper

A

-spots of mixture are placed on paper
-left to dry
-suspended in a solvent
-as solvent travels up paper - different components move up paper at different speeds
-causes OG mixture to separate into different spots = chromatogram

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4
Q

Separating monosaccharides

A

-mixtures already coloured don’t have to be stained
-spot is placed on the line - known spots are placed next to the sample
-suspended in a solvent
-unknown can be identified by comparing + matching with known solutions

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5
Q

Separating amino acids

A

-spots of unknown placed to known
-known = lysine, alanine + methionine
-paper is suspended
-separate different distances - depending on charge + size
-can be compared to known
-same distance = contain this amino acid

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6
Q

How to work out RF value?

A

-line drawn to show how far solvent has travelled from solvent front
-Rf value = how far a dissolved molecule travels during its mobile phase

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7
Q

What does a smaller Rf value mean?

A

-smaller = less soluble - larger in size

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8
Q

Rf value equation

A

-distance moved by solute/distance moved by solvent

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9
Q

Identifying chloroplast pigments

A

-carotenoids = highest value
-chlorophyll B = lower Rf
-chlorophyll A = Rf between B + C

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