Chromatography Flashcards
What is Chromatography
It is the separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase
Name the separation techniques
Chromatography
Infra red Spectrometry
Ultraviolet Spectrometry
What are the different Chromatography Techniques
Paper Thin Layer (TLC) Column Gas HPLC
The principle on which all chromatographic separation techniques are based is that….
separation of a mixture of components occurs as a result of selective absorbance of the components of the mixture on a stationary phase while carried by a mobile phase.
What spectroscopic method is used to detect heavy metals
Atomic Absorption
State the principle of ultra violet absorption spectroscopy
Absorption of UV radiation by molecules results in the promotion of electrons from their ground state energy levels to higher energy levels.
What is the principle of infrared spectrometry
molecules absorb infrared radiation of different frequencies.