Chpater 1 pp 3

Question 1

_____ is very sensitive – often used for visualizing where molecules inside a cell are localized.

Answer 1

Fluorescence microscopy

Question 2

certain proteins are naturally fluorescent such as GFP, which is a & RFP which is a ___ and are made by

Answer 2

green fluorescent protein
red fluorescent protein) are made by
jellyfish.

Question 3

The jellyfish genes can be transferred to other cells using standard

Answer 3

recombinant DNA techniques

Question 4

The proteins can be seen in the cells by fluorescence

microscopy. No need to

Answer 4

stain or fix the cells.

Question 5

Much higher resolution than light
microscopy allows us to see much
smaller objects, in the range of
nanometers

Answer 5

electron microscopy

Question 6

2 types of electron microscopy

Answer 6

scanning electron microscopy and transmission

Question 7

• electrons are bounced off of
  objects, so we see the outside
  surfaces in a 3D image.

Answer 7

Scanning electron microscopy

Question 8

• electrons pass through objects, so we

see the inside.

Answer 8

Transmission electron microscopy

Question 9

• Shines electrons on specimens.
• Specimens must be coated in metal
first, so cannot be alive.
• Much greater resolution (0.2 nm)
than light microscopy because of the
short wavelength of electrons

Answer 9

electron microscopy