Chp 19, 27 Flashcards
Viral Genomes consist of __ or __ and are classified as __ viruses or __ viruses.
ds/ss DNA, ds/ss RNA; DNA, RNA
What is a Capsid?
The protein shell that encloses the viral genome. Capsids are built from protein subunits called capsomeres.
What are viral envelopes?
Viral envelopes surround the capsids of influenza viruses and many animal viruses. They are derived from membranes of host cells and therefore contain host cell phospholipids and membrane proteins.
What are phages?
Phages are viruses that infect bacteria. They are composed of an elongated capsid head and a protein tail piece that injects the phage DNA inside.
What occurs after the viral genome enters the host cell?
The cell begins to make viral proteins. The virus makes use of host machinary. Viral nucleic acid molecules and capsomeres self-assemble into new viruses.
What are the two alternative reproductive mechanisms found in phages?
The lytic cycle and the lysogenic cycle.
What is a virulent phage?
A phage that reproduces only by the lytic cycle.
Describe the Lysogenic cycle.
This cycle replicates the phage genome without destroying the host. The viral DNA molecule is incorporated into the host cell’s chromosome.
What are Temperate phages?
Phages that use both the lytic and lysogenic cycles.
What are the three ways in which bacteria defend themselves against phages?
Natural selection, restriction enzymes, and the Crispr-Cas system.
Explain how Natural selection aids in combat against phages for bacteria.
Natural selection favors bacterial mutants with surface proteins that cannot be recognized as receptors by a particular type of phage.
What is the role of restriction enzymes?
Restriction enzymes restrict a phage’s ability to replicate within the bacterium. Foreign DNA can be identified as such and cut up by these cellular enzymes.
Both bacteria and archaea can protect themselves from viral infection with the Crispr-Cas system. What does this acronym stand for?
Clustered regularly interspaced short palindromic repeats.
What is the role of each “spacer” sequence between the repeats?
Each “spacer” sequence corresponds to DNA from a phage that had previously infected the cell.
What are the two key variables used to classify viruses that infect animals?
ss or ds DNA/RNA and the presence or absence of a membranous envelope.
What role does the Viral envelope play?
Viral glycoproteins on the envelope bind to specific receptor molecules on the surface of a host cell. The viral envelope is usually derived from the host cell’s plasma membrane as the viral capsids exit.
What make retroviruses unique?
Retroviruses use reverse transcriptase to copy their RNA genome into DNA.
What is an example of a retrovirus?
HIV, a retrovirus that causes AIDS.
Unlike a prophage, a provirus…
remains a permanent resident of the host cell.
What is a provirus?
Viral DNA integrated into the host genome.
What transcribes the proviral DNA into RNA molecules?
RNA polymerase.
What two roles does the RNA molecules play in viral infection?
RNA molecules function both as mRNA for synthesis of viral proteins and as genomes for new virus particles released from the cell.
List 3 ways viral diseases affect animals.
Viruses may damage or kill cells by causing the release of hydrolytic enzymes from lysosomes. Some viruses cause infected cells to release toxins and others have molecular components such as envelope proteins that are toxic.
Viral infections cannot be treated by
antibiotics.
What epidemic of a flu-like illness appeared in Mexico and the US in 2009?
H1N1.
Viruses with RNA genomes are more prevalent in
Plants.
Plant viruses spread disease by two major routes:
Horizontal transmission, entering through damaged cell walls, and Vertical transmission, parent to offspring.
What are Prions?
Infections proteins that appear to cause degenerative brain diseases in animals. Prions are misfolded proteins that are able to convert more proteins into prions.
What are three diseased caused by Prions?
Scrapie in sheep, mad cow disease, and Creutzfeldt-Jakob disease in humans.
Why are Prokaryotes the most abundant organisms on earth?
Prokaryotes have the ability to adapt to diverse habitats and thrive almost everywhere.
What are the three most common shapes of Prokaryotes?
Spheres (cocci), rods (bacilli), and spirals.
What is the role of the cell wall? What do most bacterial cell walls contain?
The cell wall maintains cell shape, protects the cell, and prevents it from bursting in a hypotonic environment. Most bacterial cell walls contain peptidoglycan, a network of sugar polymers cross-linked by polypeptides.
What is peptidoglycans?
A network of sugar polymers cross-linked by polypeptides.
Which domain lacks peptidoglycan?
Domain archaea.