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Chemistry Rotation Final Flashcards

1
Q

How often do you change the filter for HGB A1C QC (Tosoh)?

A

Every 400 samples

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2
Q

How often do you change the column for HGB A1C QC (Tosoh)?

A

Every 2500 samples

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3
Q

When do you change the duck bill on the DXI?

A

5,000 samples maintenance

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4
Q

When do you clean the probes on the DXI?

A

10,000 samples maintenance

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5
Q

What 3 tests are important to indicate a myocardial infarction?

A

CPK, AST, LDH

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6
Q

When will a BHCG do an automatic dilution?

A

> 1300

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7
Q

What is the formula for urine creatinine clearance?

A

C (mL/min) = (U x V)/P x (1.73)/(SA pt)

SA pt = surface area of patient
C = clearance in mL/min
V = urine volume in mL
U = urine creatinine
P = plasma creatinine

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8
Q

What does the yellow rack mean?

A

Remap

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9
Q

What does the blue rack mean?

A

Routine

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10
Q

What does the white rack mean?

A

Pre-spun

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11
Q

What does the red rack mean?

A

STAT

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12
Q

What is LIH and what do the results mean?

A

LIH = lipemic, icteric, hemolyzed
1 or 2 = slightly hemolyzed
3 or 4 = very hemolyzed; results are heavily affected and request a redraw or take from another tube of same preservative

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13
Q

What values for males and females would there be a CPK w/ MB reflex?

A

Males: CPK >180
Females: CPK >100

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14
Q

If troponin is increased, what else should also be increased?

A

MB

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15
Q

What is a pour over?

A

Increased potassium and decreased calcium due to pour over from K EDTA tube. Caused by potassium in the EDTA and calcium binding EDTA.

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16
Q

When would a direct LDL be ran instead of a calculated LDL?

A

Triglycerides >400

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17
Q

Gray vs green tops for glucose

A

Gray top is good for glucose specimens because it does not break glucose down. Contains sodium fluoride/potassium oxalate.

Green top is bad for glucose specimens because it can falsely decrease glucose.

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18
Q

What is the formula for anion gap?

A

(Na) - (Co2 + Cl)

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19
Q

What does a 0 error mean on the line?

A

Barcode error

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20
Q

What does a 7 error mean on the line?

A

Gripper error

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21
Q

What does a 340 error mean on the line?

A

AU connection error

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22
Q

What westgard rule is a single control outside 2 SD?

A

1 2s

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23
Q

What westgard rule is a single control outside 3 SD?

A

1 3s

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24
Q

What westgard rule are two consecutive control results greater than 2 SD?

A

2 2s

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25
What westgard rule is when there is a 4 SD difference between control values?
R 4s
26
What westgard rule is when there is 3 consecutive results greater than 1 SD on the same side of the mean?
3 1s
27
What westgard rule is when there are four consecutive results greater than 1 SD on the same side of the mean?
4 1s
28
What westgard rule is when there is a trend of 10 on one side of the mean?
10x
29
What specimens may not be loaded on the automated line?
Troponins, short draws, body fluids, HIV, CSF, ETOH, Cancer center specimens, urine CA/urine PHOS
30
What is the stability time for ionized calcium?
4 hours on ice. Ice required
31
What is the stability time for lactic acid?
30 min on ice. Ice required
32
What is the stability time for ABG?
30 min @ RT, 1 hour on ice
33
What is the stability time for blood pH?
stable for 30 min @ RT and on ice
34
What is the FFN test?
It assesses the risk of pre-term delivery
35
What are the four methods of measurement used by the radiometer?
Potentiometry, amperometry, optical pO2, spectrophotometry
36
What method does the osmometer use?
Freezing point depression
37
What type of analyzer is the Tosoh G8?
Ion exchange HPLC
38
Describe the acceptability of POO errors?
POO = unidentifiable peak due to hemoglobin variant. If the POO peak eluted after the AO peak (larger retention time) results are acceptable. If it eluted before, we cannot use this method
39
Critical value for ionized calcium?
<0.8 or >1.50
40
Critical value for pH?
<7.2 or >7.6
41
Critical value for lactic acid?
>4.0
42
What are the cardiac markers and what do they help assess
BNP: congestive heart failure CKMB: myocardial infarction Troponin: myocardial infarction
43
What marker is CEA?
colorectal cancer marker
44
What marker is PSA?
prostate cancer marker
45
Critical value for troponin
>0.5
46
What type of reaction does the DXI perform?
chemiluminescent immunoassay
47
Critical value for sodium
<120 or >160
48
Critical value for potatssium
<3 or >6
49
Critical value for calcium
<6.5 or >12
50
Critical value for glucose
<50 or >500
51
Critical value for magnesium
<1 or >5
52
Critical value for phosphorus
<1
53
Critical value for anion gap
>30
54
Critical value for CO2, Total
<10 or >45
55
What tests are affected by hemolysis?
Potassium, Iron, AST, magnesium, ferritin, folate, and ammonia
56
Normal range for pH; what does increased or decreased values mean?
7.35-7.45 decreased = acidosis increased = alkalosis
57
Normal range for pCO2; what does increased or decreased values mean?
35-45 decreased = alkalosis increased = acidosis
58
Normal range for pHCO3; what does increased or decreased values mean?
22-26 increased = alkalosis decreased = acidosis
59
Normal range for pO2; what does increased or decreased values mean?
80-100 increased = hyperoxygenated decreased = hypoxic
60
ROME
Respiratory opposite (opposite directions of CO2 and HCO3) Metabolic equal (equal directions CO2 and HCO3
61
What tests need a manual dilution on the AU?
Albumin, CRP, Cholesterol, Magnesium, Methotrexate, Phenytoin, Glucose, Urine calcium, Phenobarbital
62
What tests are measured in the ISE moedule?
Na, K, Cl
63
What type of lamp is on the AU 5800?
Halogen
64
When does the p24 antigen first appear? When does the HIV antibody first appear?
p24 --> within first 2 weeks of HIV infection HIV antibody --> 23 to 90 days post exposure
65
HBsAg
Hepatitis B surface antigen - first seriological marker after infection with HBV; will go away if acute
66
Anti-HBc
Hepatitis B core antibody Total - appears after HBsAg and indicates current/previous exposure to virus
67
Anti-HBs
Hepatitis B surface antibody - can indicate previous exposure to HBV or HBV vaccination
68
What is positive that is consistent with a previous hepatitis B infection?
Hep B surface antibody (Anti-HBs) Hep B core antibody Total (Anti-HBc)
69
What is positive that is consistent with a Hep B vaccine?
Only Hep B surface antibody (Anti-HBs)
70
What is positive that is consistent with a chronic Hep B infection?
Hep B surface antigen (HBsAg) Hep B Core Antibody Total (Anti-HBc)
71
What is a normal hepatitis profile?
Negative for everything :)
72
What type of reaction does the Architect perform?
Immunoassay --> chemiluminescent microparticle
73
What are the names of the immunosuppressant drugs ran on the Architect?
Tacro, Siro, and Cyclo
74
What is CCP?
A marker for rheumatoid arthritis

Chemistry (12 decks)

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