Chemistry Review. Flashcards
Difference between serum and plasma?
Serum doesn’t have fibrinogen
How do you get serum?
Collect in red top tube, sit, pipette off top
How long does blood sit to clot for serum?
20-30 minutes
How do most anticoagulants work?
Combine with calcium
Order of collection tubes?
Blue, red, tiger top, green, lavender, gray
2ml of whole blood = ___ of serum/plasma
1
You should draw enough blood to run each test ___ times
3
BUN is also called?
Blood urea nitrogen
Blood urea nitrogen is from the breakdown of what?
Protein
What is azotemia?
Increase in BUN
Creatinine is from the breakdown of what?
Muscle
Creatinine is excreted at a ____ rate
Constant
Do not run a water deprivation test on…?
Dehydrated patient or a patient with azotemia
URINE protein: creatinine is a confirmation test for what?
Protein loss through kidney
4 kidney tests are called…?
BUN, Creatinine, Water deprivation, and urine protein creatinine
Three functional tests of the liver are what?
Bilirubin, bile acids, and TP
Bilirubin is from the breakdown of what?
Hemoglobin
Where is bilirubin conjugated?
Liver
Bilirubin is a component of what?
Bile
What will break bilirubin down and at what rate?
UV Light, 50% per hour
Bile acids are made where?
Liver
Bile acids are stored in what?
Gallbladder
Bile acids are excreted into what?
Intestines
How do you collect for serum bile acid tests?
Fast animal for 12h, collect 1ml of serum, feed animal high fat meal, wait 2h, collect 1ml of serum
Most protein is formed by the _____.
Liver
What 2 proteins are measured with a serum TP test?
Albumin and globulin
TP is most commonly increased because of what?
Dehydration
What are the 4 damaged hepatocyte tests?
ALT, AST, ID(SD), GLDH