Chemistry Review. Flashcards

1
Q

Difference between serum and plasma?

A

Serum doesn’t have fibrinogen

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2
Q

How do you get serum?

A

Collect in red top tube, sit, pipette off top

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3
Q

How long does blood sit to clot for serum?

A

20-30 minutes

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4
Q

How do most anticoagulants work?

A

Combine with calcium

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5
Q

Order of collection tubes?

A

Blue, red, tiger top, green, lavender, gray

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6
Q

2ml of whole blood = ___ of serum/plasma

A

1

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7
Q

You should draw enough blood to run each test ___ times

A

3

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8
Q

BUN is also called?

A

Blood urea nitrogen

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9
Q

Blood urea nitrogen is from the breakdown of what?

A

Protein

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10
Q

What is azotemia?

A

Increase in BUN

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11
Q

Creatinine is from the breakdown of what?

A

Muscle

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12
Q

Creatinine is excreted at a ____ rate

A

Constant

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13
Q

Do not run a water deprivation test on…?

A

Dehydrated patient or a patient with azotemia

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14
Q

URINE protein: creatinine is a confirmation test for what?

A

Protein loss through kidney

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15
Q

4 kidney tests are called…?

A

BUN, Creatinine, Water deprivation, and urine protein creatinine

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16
Q

Three functional tests of the liver are what?

A

Bilirubin, bile acids, and TP

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17
Q

Bilirubin is from the breakdown of what?

A

Hemoglobin

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18
Q

Where is bilirubin conjugated?

A

Liver

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19
Q

Bilirubin is a component of what?

A

Bile

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20
Q

What will break bilirubin down and at what rate?

A

UV Light, 50% per hour

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21
Q

Bile acids are made where?

A

Liver

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22
Q

Bile acids are stored in what?

A

Gallbladder

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23
Q

Bile acids are excreted into what?

A

Intestines

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24
Q

How do you collect for serum bile acid tests?

A

Fast animal for 12h, collect 1ml of serum, feed animal high fat meal, wait 2h, collect 1ml of serum

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25
Q

Most protein is formed by the _____.

A

Liver

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26
Q

What 2 proteins are measured with a serum TP test?

A

Albumin and globulin

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27
Q

TP is most commonly increased because of what?

A

Dehydration

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28
Q

What are the 4 damaged hepatocyte tests?

A

ALT, AST, ID(SD), GLDH

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29
Q

ALT is not used in what?

A

Large animal

30
Q

ID(SD) replaces _____ in what animals?

A

ALT; large animals

31
Q

GLDH is used in what animals?

A

Cattle , sheep, goats

32
Q

What are the two Cholestasis tests of the liver?

A

AP and GGT

33
Q

AP is elevated in young animals, why?

A

Bone growth

34
Q

GGT is a good indicator for what dz?

A

Small animal fatty liver dz

35
Q

3 types of liver tests are?

A

Functional, damaged hepatocyte, and cholestasis

36
Q

The two types of pancreatic tests are what?

A

Endocrine and exocrine

37
Q

Endocrine pancreatic test includes what 2 things?

A

Glucose and fructosamine

38
Q

What hormone lowers blood glucose?

A

Insulin

39
Q

What hormone raises blood glucose?

A

Glucagon

40
Q

How do you perform a glucose curve on an animal receiving insulin BID?

A

q2h for 8-12h…mock normal day

41
Q

What increases the amount of fructosamine?

A

Chronic hyperglycemia

42
Q

Fructosamine gives you and idea of blood glucose levels over what period of time?

A

1-2 weeks

43
Q

Exocrine pancreatic tests include what 3 things?

A

Amylase, lipase, trypsin

44
Q

Amylase breaks down what?

A

Starches and glycogen

45
Q

Lipase breaks down what?

A

Long chain fatty acids

46
Q

Trypsin breaks down what?

A

Protein

47
Q

Trypsin test of choice?

A

Serum trypsinlike immunoreactivity

48
Q

Of the pancreatic exocrine tests, which one can be measured by IDEXX SNAP test?

A

Lipase

49
Q

The endocrine system contains what 3 tests?

A

Thyroid, adrenocortical function, and high-dose dex suppression

50
Q

The thyroid portion of the endocrine system tests includes what 2 things?

A

Total T4 and Cholesterol

51
Q

Total T4 screens for what in cats? Dogs?

A

Hyperthyroidism; hypothyroidism

52
Q

What is the confirmation test of Total T4 called?

A

Free T4

53
Q

Cholesterol is made by the what?

A

Liver

54
Q

Cholesterol is usually increased with what?

A

Hypothyroidism

55
Q

Hypoadrenaocroticism is also know as?

A

Addisons

56
Q

Hyperadrenocorticism is also known as?

A

Cushings

57
Q

Low pre and post sample on ACTH stim test =

A

Addisons

58
Q

High post sample on ACTH Stim test =

A

Cushings

59
Q

High results at 4 and 8 h on low dex suppression =

A

Cushings

60
Q

Adrenal tumor =

A

No suppression of cortisol

61
Q

Pituitary tumor =

A

Cortisol suppressed

62
Q

Na

A

Natremia

63
Q

K

A

Kalcemia

64
Q

Cl

A

Chloremia

65
Q

What does HCO3 do?

A

Blood buffer

66
Q

HCO3 is estimated from?

A

Total CO2

67
Q

Ca has an inverse relationship with what

A

Phosphorus

68
Q

Mg

A

Magnesemia

69
Q

Ca

A

Calcemia

70
Q

P

A

Phosphatemia

71
Q

When do you see an increase in Anion Gap?

A

Lactic acidosis and acetoacidosis