Chemistry Chromatogrophy Flashcards
What is the aim
To investigate how paper chromatography can be used to separate snd tell the difference between coloured substances, including the calculation of Rf values
What is paper chromatography used for
A method to separate coloured substances.
What will you do in this practical
apply a small sample of a mixture, such as ink or plant extract, near the bottom of a strip of chromatography paper. When the lower end of the paper is immersed in the solvent, the solvent will rise up the paper. As it moves, the different substances in the sample will separate. By observing the distinct bands that form, the composition of the mixture can be analysed.
What is the purpose of paper chromatography
To separate and differentiate between coloured substances
What should be done if the water solvent does not travel three quarters of the way up the paper
Wait longer for the solvent to travel
What is the purpose of drawing another pencil line on the dry part of the paper
To mark the solvent front line
What is the significance of the solvent front line in paper chromatography?
It helps calculate the Rf values