Chemistry Flashcards
1
Q
What determines physico-chemical properties of proteins?
A
2
Q
Ionisation states of the histidine side-chain
A
3
Q
What is post-translational modifications?
- types of PTM
A
4
Q
Consequences of ionisable groups
A
5
Q
Define isoelectric point
- what determines protein’s ionisation state
- solubility of protein @ isoelectric point
A
6
Q
How does isoelectric point affect insulin?
A
7
Q
How can we separate proteins?
A
8
Q
What is Gel electrophoresis?
- How can proteins be separated in this method?
- Where do different ions migrate to?
- Structure of analyte
A
9
Q
PAGE
A
10
Q
SDS-PAGE
A
11
Q
Protein detection methods post-electrophoresis
A
12
Q
Methods to measure protein concentration
A
13
Q
Methods to analytically analyse proteins
A
