Chemical Identification of Food Molecules - Lab 4 Flashcards
Chemical indicators are:
chemical solutions that react with particular food molecules, then they change their colors indicating the presence of particular food
molecule.
The identification of food molecules may be:
Qualitative or quantitative
Qualitative
detect the presence or absence of the food molecule(s)
Quantitative
Detect the amount of the nutrient(s).
The chemical tests used to detect food molecules in
this laboratory:
are ONLY qualitative tests.
Molisch’s reagent
Detects all types of carbohydrates.
Biuret reagent
Detects all types of proteins
Emulsification test
Detects all types of lipids
Molisch’s test positive test is indicated by:
Violet of purple ring
Bile
A natural emulsifier produced by the liver
Artificial emulsifiers
Ethanol, tween, liquid soap, and sodium carbonate
Positive lipid test results in a
milky emulsification
Positive protein tests results in a
violet color (biuret test)
Positive Carbohydrate test results in
Redish-violet ring
(Molisch’s test)
biuret solution/reagent consists of
Copper sulfate and sodium hydroxide