Cell Cycle - Lab 5 Flashcards

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1
Q

Most Somatic cells:

A

Divide for growth and repair.

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2
Q

The stages that a cell passes through from the time it is formed until it divides is called:

A

The cell cycle

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3
Q

The cell cycle includes two main phases:

A

Interphase and Mitotic/meiotic

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4
Q

Interphase:

A

The longest phase in the cell cycle

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5
Q

Mitotic and meiotic

A

Somatic cell and germ cell
the M-phase

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6
Q

Interphase stages:

A

G1 phase (gap 1)
S phase (synthesis phase)
G2 phase (gap 2)

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7
Q

In the G1 phase, the cell is:

A

Growing in size

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8
Q

In the S phase, the cell:

A

Replicates/duplicates its DNA and replicate the centriole.

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9
Q

Duplicated chromosomes are held together by a structure called:

A

centromere

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10
Q

In the G2 phase, the cell is:

A

Undergoes routine metabolism and continues to grow

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11
Q

Microscopic appearance of the Interphase stage of
the animal and plant Cells (metabolic active cell):

A

Chromatin is dispersed
one or more nucleus is evident
transparent nuclear membrane

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12
Q

M phase:

A

Mitosis or meiosis

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13
Q

Mitosis

A

somatic cell

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14
Q

Meiosis

A

germ cell

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15
Q

Types of division that take place during mitosis

A

Cytokinesis and karyokinesis
(Cytoplasmic division and nucleus division)

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16
Q

Mitosis is divided into 4 stages:

A

Prophase
metaphase
anaphase
telophase

17
Q

Prophase + light microscopic characteristics:

A

Longest phase of mitosis
Duplicate centioles move towards opposite poles of the cell (not seen by LM)
Chromosomes shorten and thicken (seen by LM)
nuclear membrane and nucleolus disappear. (seen
by LM)
Mitotic spindle start to from by the microtubules asters of
the centrioles

18
Q

Metaphase stage:

A

Condensed chromosomes line up along the equator by their centromeres to the spindle fibers

19
Q

Anaphase:

A

Chromosomes separate from each other (Break at centromere) and pulled towards opposite poles of the cell.

20
Q

Telophase

A

separated chromosomes start to form clear new nuclei in the newly formed daughter cell.
Spindle fibers disappear
Cytokinesis occurs

21
Q

Animal Cell cytokinesis:

A

Cell membrane pinches in
*Cleavage furrow & Contractile ring

22
Q

Plant Cell cytokinesis:

A

New cell wall Golgi vesicles form between two
nuclei to form the 2 new daughter cells separated by cell wall.
*Cell wall Golgi vesicles. -> Cell plate

23
Q

G0 phase:

A

cellular state outside of
the replicative cell cycle.
Resting phase.

24
Q

Cells that may stay in the G0 stage temporarily and
then inter the mitosis stage again are called:

A

quiescent cells

25
Q

Cells that may stay in the G0 stage permanently and
don’t inter the mitosis stage again are called:

A

senescent cells undergoing ageing and DNA damage

26
Q

meristem

A

Embryonic tissue where mitosis is occurring.

27
Q

elongation zone

A

Cells are long rectangles.
they are not undergoing mitosis.

28
Q

Onion root-tip cells have a cell cycle of:

A

24-hours (approximately)

29
Q

Mitosis can be studied in:

A

Onion or garlic root tip, animal cells of
tadpole tail, Fish blastula and epithelial cells of Fish
gills

30
Q

Stain used in the onion root tip experiment: (mitosis)

A

Acetocarmine

31
Q

The mitotic index is:

A

a measure of the growth
rate of a cell population

32
Q

The mitotic index may be elevated during

A

Normal growth
cancer cells
Cellular repair (wound healing)

33
Q

To calculate mitotic index

A

Cells in mitosis/total cells

34
Q

Materials used in onion root tip experiment:

A

Microscope with 40x, 100x, and 400x.
Prepared microscope slide of an onion root tip.
Worksheet for mitosis and mitotic index.