Animal Part - Lab 6 Flashcards
The cell membrane (plasma membrane), is composed of:
A bilayer of phospholipid molecules with a mosaic of proteins molecules embedded or attached to it
phospholipids and proteins are necessary for
the semipermeable cell membrane to:
To maintain the integrity of the cell contents
Separate the inside of the cell from the outside environment.
Allow selected substances to pass through it.
The effect of heat and alcohol damage of cell
membrane can be tested on either the phospholipids
or proteins components by using:
Beet Root Plant
Tonoplast
Contains a water soluble pigment Betacyanin (75%) and anthocyanin (25%)
Beet characteristic color
Purple to pink
How to Detect The Leaked Pigment?
can be observed qualitatively by eye or measured quantitatively by using the colorimeter.
What size to cut the beetroot pieces?
1cm
What temperatures did we use to study The Effect of Heat on Release of
Pigments From Beet Plant?
0, 20, 40, 60, 80 (celcius)
Percentage of ethyl used to
study the Effect of Organic solvents like alcohol on the
Permeability of Biological Membranes.
0, 10, 30, 50, 70, 90
Incubating the pieces of beetroot at increasing
concentrations of alcohol will:
damage and dissolve
the biological membranes lipids and thus it
permeabilize it. (red pigment will leak out of the cell membranes)
Incubating the pieces of beetroot at temperatures up to
40 Celsius would:
increases the kinetic energy of the cell membrane. (red pigment will leak out of the cell membranes)