Chemical Food Tests Flashcards
reagent in Benedict’s test
Benedict’s solutioin
colour change in positive test for reducing sugar
blue to brick red
describe the test for reducing sugars
add Benedict’s solution to a sample
heat the solution
explain why a non-reducing sugar test can only be done on a solution which has tested negative for reducing sugars
the test may be positive for the monosaccharides that are already in the solution rather than the disaccharides that have been hydrolysed
describe the test for non-reducing sugars
- add hydrochloric acid to a solution that has tested negative for reducing sugars and heat to hydrolyse into monosaccharides
- add sodium-hydrogencarbonate to the sample to neutralise it
- add benedict’s solution and heat
describe the test for starch and result if positive
add iodine to the sample
if contains starch, will change from yellow to blue-black
reagent in test for proteins
biuret reagent
does the test for proteins require the solution to be heated?
no
colour change if proteins are present
blue to light purple
name of reagent in emulsion test for lipids
ethanol
does the test for lipids require the solution to be heated?
no
negative result for lipids
no emulsion will form
positve result for lipids
an emulsion will form
describe the test for lipids
- add ethanol to a test tube containing the sample
- shake the test tube
- and the solution to water
describe the test for proteins
- add three drops of biuret solution to the test tube containing the sample