Chemical Examination Flashcards
Defined as test using different reagents
Confirmatory testing
It currently provide a simple, rapid means for performing medically significant chemical analysis of urine.
Reagent strips
pH of normal random samples
4.5 - 8.0
pH of morning specimen
5.0- 6.0
Principle of pH
Double indicator
Double indicator system of pH
Methyl red and bromthymol blue
Color in reagent strip if urine is acidic
Yellow
Color in reagent strip if urine is alkaline
Blue
Principles of protein
Protein errors of indicators
Early renal disease
Protenuria
Major serum protein found in normal urine
Albumin
It is not indicative of actual renal disease
Prerenal protenuria
Increased serum protein levels is the excretion of what ?
Bence Jones protein
True renal disease
Renal protenuria
Positive indication of protein
green
Principle of glucose
Double sequential enzyme reaction
Positive indication of glucose
Brown color
Pass through phenomenon
Clinitest
Principles of ketones
Sodium nitroprusside
Positive indication of ketones
Purple
Used for doubtful reagent strip result
Acetest (acetest tablet)
Principle of blood
Pseudoperoxidase
Positive indication of blood
blue color
Principle of bilirubin
Diazo reactions
Confirmatory test for bilirubin
Ictotest (ictotest tablet)
Principles of urobilinogen
Ehrlich’s aldehyde reaction and diazo reaction
Positive indication of bilirubin
Violet
Positive indication of urobilinogen
Red
Principle of nitrite
Greiss reaction
Positive indication of nitrite
Pink
Principle of leukocytes
LE test
Positive indication of leukocytes
Purple