Chem Flashcards
Protein occur in a variety or diseases esp. ….
Liver and kidney
Plasma proteins produced by
Liver and immune system
It’s composed of reticulo- endothelial tissues, lipid tissues, and plasma cells
Plasma protein function
Helping form the structural matrix of all cells, organs, and tissues
Maintains osmotic pressure
Serving as enzymes for biochemical research
Serve as hormones
Function in blood coagulation
Buffers in acid-base balance
Defending body against pathogenic microorganism
Transport carrier of molecule of most constituents of plasma
Protein Assay includes
Total protein, albumin, fibrinogen
Globulins/ albumin/globulin ratio
What alters TP?
Hepatic system
Altered protein distribution
Altered protein breakdown or excretion
As well as DEHYDRATION and HYDRATION
Hydration alters
TP
TP especially tells
State of hydration
A dehydrated animal has a relatively ….
Hydrated….
Elevated total protein value (hyperproteinemia)
Over hydrated- low tp (hypoproteinemia)
TP are used in initial screening test for patients with
Edema, ascites, diarrhea, weight lost, renal and hepat disease, and blood clotting problems
TP
Refraction enter measures
Refractive index of serum or plasma
(which is a function of the concentration of solid particles in the sample)
Proteins are primary solid in plasma
What makes the refractometer so good?
Inexpensive, accurate, and fast
The 2nd method for getting TP is the biuret method measures
The # of molecules containing more then 3 peptide bonds in plasma or serum.
Hypoproteinemia is most likely caused by
Albumin loss
Globulins are responsible for
Iron transport, heme binding, and fibrin formation
And lysis
Major heptatobiliary Assays
Enzymes released from damaged hepatocyte
Enzymes associated with Cholestasis
Hepatocyte function tests
