Characteristics

Question 1

one of the major features of bacteria is their appearance following growth on various
1.
2.
3.

Answer 1

1. Color
2. Abundance of growth
3. Color or culture

Question 2

SIZE:

punctiform
small
medium
large
which one is smaller
which one is bigger

Answer 2

<1mm
1-2mm
3-4mm
>5mm
gram positive
gram negative

Question 3

_____: edge of colony

1. Smooth
2. resembles roots but no branching
   - aka
   - example
3. Rough
   - example
4. Usually in fungi
5. do not follow any shapes; margin is uneven and spread-out
6. appears wavy or curled under microscope
7. phenomenon where there’s hazy blanket of growth on surface
   - examples

Answer 3

Form/ Margin

1. Smooth
2. filamentous
   - Medusa’s head
   - Bacillus anthracis
3. Rough
   - Diphtheroids
4. rhiziod
5. irregular
6. curled/ crenated
7. Swarming
   - Proteus mirabilis, Proteus vulgaris

Question 4

Elevation
1. colonies lie evenly in the agar and no elevation
2. slightly raised but the top is still flat
3. dome-shaped and top is smoothly curved
4. slightly elevated and there’s a bump in a center
5. opposite of umbonate as in the center it is depressed

Density: thru ____
1. very clear
2. allows some light to pass thru the colony
3. does not allow any light to pass thru

Answer 4

1. flat
2. raised
3. convex
4. umbonate
5. umbilicate

illumination
1. Transparent
2. Translucent
3. Opaque

Question 6

_____: describe particular genus

1. white
2. gray
3. yellow
4. ____

Answer 6

Color

1. Staph except aureus
2. Enterococcus
3. Micrococcus & Neisseria
4. Buff

Question 7

_____: inherent characteristic for
these specific types of organism that is confined generally in their colony

1. green = ______ in BAP
2. green = ______ in EMB
3. brick red
4. blue
5. purple
6. brown black

Answer 7

Pigment

1. P. aeruginosa
2. E. coli
3. Serratia marcescens
4. Kluyvera spp
5. Chromobacterium violaceum
6. Prevotella melaninogenica

Question 8

Odor
1. old sock
2. fruity/grape
3. putrid
4. musty
5. freshly plowed field

Answer 8

1. S. aureus
2. P. aeruginosa
3. P. mirabilis
4. Haemophilus spp.
5. Nocardia spp.

Question 9

Gas

1. helps to facilitate the observation of gas production
2. cracking or bubble formation medium

Answer 9

1. Durham tube
2. TSIA

Question 10

Hemolysis

1. complete lysis
   - zone of hemolysis is larger than the colonies
   - zone of hemolysis is smaller than the colonies (aka _____)
2. partial lysis of RBCs
   - organism
   - color
3. no lysis
4. there’s complete hemolysis surrounded by a zone of partial hemolysis
   - organism

Answer 10

1. Beta
   - streptococcus pyogenes
   - streptococcus agalactiae (aka bull’s eye colony)
2. Alpha
   - streptococcus pneumoniae
   - green
3. Gamma
4. Alpha prime
   - viridans