Chapter 9 Flashcards
1
Q
How to sequence similar searches to assign gene function
A
- BLAST (basic local alignment search tool)
- PCR (polymerase chain reaction)
2
Q
BLAST
A
- similarities reflect evolutionary relationship and shared functions
3
Q
PCR
A
- fast technique to amplify DNA segments –> molec photocopying
- diagnosis of disease–> detection D or bacterial or viral
- DNA forensics/paternity
- DR.KB Mullis
4
Q
Components of PCR
A
- two primers(for each strand)
- DNA poly (Taq polymerase)
- dNTP
- Buffer solution: proives needed pH and [salt]
- MgCl2 :activation function
- DNA template
5
Q
PCR Process/CYCLE
A
- thermocycler
- denature/melt @high
- annealing: allow primer to anneal to template @low
- extention/elongation : exist @middle
6
Q
PCR amplification of DNA correctly is dependent on :
A
- primer sequence+length
- uniqueness of primer
- amplification conditions:temp,concentration,[MgCl2]
- primer design= accuracy
7
Q
Template DNA for PCR
A
- genomic
- plasmid
- low or partially degraded samples–>sensitive/exponential
- PCR amplified DNA
8
Q
Reverse Genetics
*types
A
- find associated phenotype for a genotype (experimental)
- RNA Interference: silence gene expression
- Gene Knockout: disrupt gene on chromo
- > yeast:PRC
- > depends on homologous recombination
