Chapter 8: DNA Technology

Question 1

Restriction enzymes

Answer 1

Cut DNA based on target sequence

Question 2

DNA Ligase

Answer 2

Enzyme

Seals or lighted DNA pieces together

Question 3

Making Recombinant DNA by use of R. Enzyme and Ligase

Answer 3

• restriction enzyme cuts DNA
• addition of DNA fragment from another source (stick by base pairing)
• fragment produced by same restriction enzyme
• one possible combo
• DNA ligase seals strand

Question 4

Cloning

Answer 4

• isolate gene of interest
• cut vector with R.E. for compatible strands
• Mix2 DNA’s together, ligate
• transform
• screen

Question 5

Goals

Answer 5

Eliminate undesirable phenotypic traits

Combine beneficial traits to make new organisms

Create organisms that synthesize product for human needs