chapter 8 - DNA, genes, and protein synthesis Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What is a gene

A

A gene is a section of DNA that contains the coded information for making polypeptides and functional RNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Scientist discovered that there must be three bases that code for each amino acid suggest how they came to this conclusion

A

Only 20 different amino acid’s occur in proteins

Each amino acid must have its own code of bases on the DNA

There are only four different bases in DNA

4 to the power of 3 = 64. which is enough to satisfy the requirements of 20 amino acids.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is a degenerate code

A

This means that more than one triplet codes for the same amino acid this reduces the number of mutations.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is meant by non-overlapping

A

Each triplet is only read once and the triplets do not share any bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Where is DNA located in eukaryotes

A

In eukaryotic cells DNA molecules are found in the nucleus. They are long and linear and associated with proteins called histones To form structures like chromosomes. Mitochondria and chloroplasts contains DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is DNA represented as in prokaryotic cells

A

In prokaryotic cells DNA is short and circular it is not associated with proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are homologous chromosomes

A

Chromosome pairs that are approximately the same length, same centromere position, and genes with the same corresponding Loci position. However they can carry different alleles of the same gene.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is an allele

A

An allele is described as an alternate form of a gene with every gene existing in two or more possible forms. Each individual inherits two alleles of every gene from their parents.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How is a chromosome formed

A

The DNA is combined with histones, a DNA-histone complex is coiled. Coils fold to form loops and loops coil and pack together to form the chromosome.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is a Genome

A

It is the complex set of genes in a cell including mitochondria and all chloroplasts DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what is a proteome

A

The full range of proteins produced by a genome. This is sometimes called the complete proteome. refers to the proteins produced by a given type of cell under certain conditions.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the structure of RNA

A

Ribonucleic acid (RNA) is a polymer made up of repeating mononucleotide subunits. It’s forms a single strand in which each nucleotide is made up of a pentose sugar (ribose) one of the organic bases and a phosphate group.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Describe the structure of transfer RNA (TRNA)

A

relatively small molecule made up of around 80 nucleotides. It is a single-stranded chain folded into a cloverleaf shape. At one end there is the point of attachment of an amino acid and at the other end there is an anticodon loop.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Define transcription

A

A complimentary section of part of the sequence is made in the form of a molecule called pre-mRNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Define translation

A

The mRNA is used as a template to which complimentary tRNA molecules attach amino acids and are linked together to form a polypeptide.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What occurs in transcription

A

DNA helicase splits the DNA strand. The template strand is used, and complimentary nucleotides from the pool present in the nucleus are joined together to form a pre-mRNA molecule RNA polymerases joins these bases together. adenine binds to uracil not thymine. when RNA polymerase reaches a stop codon it detaches and the production of mRNA is complete.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What happens in splicing of pre-mRNA

A

In prokaryotic cells transcription results in the production of mRNA from DNA. In eukaryotic cells transcription occurs and results in the production of pre-mRNA which is then spliced to form mRNA. Exons are spliced together and introns are removed from pre-mRNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Describe the process of translation

A

Once the mRNA has diffused out of the nucleus through the nuclear pore. ribosomes attach to the mRNA ready for translation. A ribosome attaches to a starting codon (AUG) at one end of the mRNA. A tRNA molecule with an anti codon (UAG) pairs with the code on on the mRNA. The tRNA carries A specific amino acid in this case it is (methionine) the ribosome moves along the mRNA bringing together to tRNA molecules at any one time. the amino acids are joined by a peptide bond Using an enzyme and ATP. Once the third tRNA molecule binds, the first tRNA molecule is set free to collect another amino acid from the pool. up to 50 ribosomes can pass behind the first so polypeptides can be assembled simultaneously. synthesis stops when ribosome reaches a stop codon.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Assembling a protein after translation

A

Sometimes a single polypeptide is a functional protein. Often a number of polypeptides are linked together to give a functional protein (quaternary structure)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is an exon

A

Coding regions of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What is an intron

A

Non-coding regions of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

what is the definition of a mutation

A

Changes to the DNA that can arise spontaneously during the replication and changes The base sequence or quantity of DNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is a missense mutation

A

When the amino acid is changed during a mutation

24
Q

What is a silent mutation

A

When the amino acid does not change or the bases code for the same amino acid.

25
Q

What is a nonsense mutation

A

When the amino acid is changed to a stop amino acid

26
Q

What is substitution

A

When one nucleotide in the DNA sequence is replaced by another, this can either cause a nonsense, misense or a silent mutation.

27
Q

What is insertion

A

When one or more nucleotide pairs are inserted into the sequence. This causes a frame shift to the right

28
Q

What is deletion

A

When one or more nucleotide pairs or deleted from the sequence. This causes a frame shift to the left

29
Q

How often does the mutation occur

A

One in every 100 million bases

30
Q

What is a small scale mutation

A

gene mutation

31
Q

What is a large scale mutation

A

Chromosome mutations, these can Have whole areas of chromosomes mutated

32
Q

Causes of mutations

A

In DNA replication DNA polymerases can add the wrong nitrogenous base to the DNA polymer. Chromosome mutations are changes to the structure or number of whole chromosomes this normally happens in meiosis.

33
Q

Inheritance of mutations

A

If a mutation occurs during the formation of gametes then it may be inherited by the offspring. This is important for evolution as it increases genetic variation within a species. If a mutation occurs during mitosis of somatic cells (body cells) then it won’t be inherited by offspring.

34
Q

What is a mutagen

A

A chemical or physical biological agent that’s cause mutations

Physical-ionising radiation
Chemical-diminishing agents, creams
Biological-viruses (insert viral DNA into the host cell)

35
Q

What is a carcinogen

A

It is a cancer mutagen

Many mutagens can cause mutations in DNA that lead to the host cell becoming cancerous

36
Q

What is a mutagenic agents and give examples

A

They increase the frequency/likelihood of mutations

Physical or chemical agents, include high energy radiation exposure, chemicals that alter DNA structure or interfere with the transcription.

37
Q

What is a proto oncogene

A

Stimulate appropriate cell division

38
Q

what is an oncogen

A

A mutation from proto-oncogens and can lead to uncontrollable cell division

39
Q

What are tumour suppressor genes

A

Tumour suppressor genes inhibit cell division, they maintain normal cell division and prevent tumours from developing if these genes become unactivated a tumour can develop.

40
Q

Why is meiosis important

A

Meiosis insures that haploid gametes are formed. Therefore they can be combined during fertilisation

41
Q

meiosis 1 early prophase 1

A

Chromosomes condense, they become shorter and fatter. Spindle fibres start to form and the nuclear membrane breaks down

42
Q

meiosis 1 late prophase 1

A

Spindle fibres are now fully formed, and spread out between centrioles.

43
Q

meiosis 1 metaphase 1

A

Pairs of chromosomes line up on the equator the orientation of maternal and paternal chromosomes is random. The pairs of chromosomes could orientate in different ways

44
Q

meiosis 1 anaphase 1

A

Maternal and paternal chromosomes move apart and are pulled separately by contraction of the spindle fibres. Each chromosome is attached to one spindle fibre at its centromere. They moved to opposite poles resulting in different combinations of maternal and paternal chromosomes to opposite poles. This is known as independent segregation

45
Q

meiosis 2 prophase 2

A

Now the cells are haploid. Chromosomes do not condense at the end of meiosis one, each chromosome still has two chromatids and spindle fibres form again

46
Q

meiosis 2 metaphase 2

A

Chromosomes line up on the spindle fibre equator independently

47
Q

meiosis 2 anaphase 2

A

Sister chromatids are separate on the spindle. each cell will receive a copy of each chromosome type (it will receive different chromatids) the genes on different chromosomes also undergo independent segregation introducing greater variation

48
Q

meiosis 2 telophase 2

A

4 haploid gametes are produced

49
Q

what are the chromosomes called once they have been crossed over

A

Recombinance chromosomes

50
Q

What is the function of the centromere

A

Spindle fibres attach to the centromere to pull apart chromatids in anaphase.

51
Q

What is crossing over

A

Homologous chromosomes crossover at points called Chaisma. The chromosomes break and rejoin exchanging some genes and therefore alleles. This recombination results in genetic variation. It occurs during PROPHASE 1

52
Q

What is random fertilization

A

One sperm cell can fuse with any egg cell. there is a random/unique combination of genes in a diploid cell

53
Q

What is independent segregation/assortment

A

When cells divide during meiosis, homologous chromosomes are randomly distributed during ANAPHASE 1
Separating independently of each other, this results in gametes having a unique combination of chromosomes.

54
Q

Why is there a lower abundance of recombinant chromosomes

A

Because crossing over does not occur that often and only a small number of sections cross over

55
Q

formula for the number of possible combinations of chromosomes

A

2 to the power n

n being the number of chromosome in a cell. eg in humans it is 23.

2 to the power of 23 = 8,388,608 Genetically unique gametes through independent assortment alone.