Chapter 8 : Antibody detection and Identification

Question 1

What is the purpose of forward type

Answer 1

To detect A or B antigens on RBC’s

Question 2

What is the purpose of reverse type

Answer 2

To detect expected A or B antibodies in plasma

Question 3

What is the purpose of Antibody screen

Answer 3

To detect unexpected antibodies.

Question 4

By performing an IAT procedure what do we use and test for?

Answer 4

The patient’s plasma or serum against reagent screening RBCs to detect unexpected antibodies

Question 5

IgG- 37 can be detected in what phase?

Answer 5

AHG

Question 6

IgM can be detected in what phase?

Answer 6

IS phase

Question 7

Is IgG clinically significant or not

Answer 7

Clinically significant

Question 8

Is IgM clinically significant or not

Answer 8

Not clinically significant

Question 9

Antibody screen is performed by

Answer 9

Incubating patient plasma with reagent RBC’s with known antigens on the cells (LISS)

Question 10

What temp and phase does IgG react at

Answer 10

37 and AHG

Question 11

Clinically significant antibodies are associated with what?

Answer 11

Hemolytic transfusion reactions and HDFN

Question 12

Screening cells are what

Answer 12

Single or pooled donor group O cells

Question 13

What is autocontrol

Answer 13

tests a patient’s serum with his or her own RBC’s

Question 14

This is used to determine if antibody is directed against patients own RBC’s

Answer 14

DAT

Question 15

Used in antibody detection and identification to enhance an antigen-antibody reaction

Answer 15

Potentiators

Question 16

What does positive auto control indicates

Answer 16

Autoantibody

Question 17

What does a negative autocontrol indicates

Answer 17

Alloantibody

Question 18

Anti-Lea, Anti-Leb, Anti-M, Anti- N, Anti-I, and Anti-P1 are what type of antibodies

Answer 18

IgM

Question 19

Which antibodies show stronger dosage

Answer 19

K, D, E, e, C, c

Question 20

Duffy, kidd, S/s shows strong or weak dosage

Answer 20

weak dosage

Question 21

What antibodies are destroyed by enzymes

Answer 21

Duffy and MNS

Question 22

What antibodies are enhanced by enzymes

Answer 22

Rh, Kidd, and Lewis

Question 23

Which antibodies are high-frequency

Answer 23

I, H, P, P1, PP1Pk

Question 24

High-titer, low-acidity antibodies are what kind of antibodies?

Answer 24

High-frequency antigens that react weakly and react at AHG phase

Question 25

IgM antibodies that react during IS crossmatching and sometimes at 37C.

Answer 25

Cold alloantibodies

Question 26

usually react with all reagent and self and donor RBCs, regardless of the antigens present

Answer 26

Autoantibodies

Question 27

What technique is usually performed to remove autoantibodies

Answer 27

Adsorption

Question 28

Anti-I, anti-H, and anti-IH are what type of autoantibodies

Answer 28

Cold autoantibodies

Question 29

To identify antibodies when the DAT is positive, the IgG must be detached by using what?

Answer 29

Elution technique

Question 30

The recovered antibody is called

Answer 30

eluate