Chapter 7 - Control of Microbial Growth Flashcards

1
Q

What kills all microorganisms?

A

Sterilization

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2
Q

What is disinfection?

A

Destruction of pathogenic organisms on an inert surface

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3
Q

What is a danger of disinfection?

A

It can damage living tissue

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4
Q

What is Antisepsis?

A

The destruction of pathogenic organisms on living tissue

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5
Q

What is sanitizer?

A

A product that lowers the number of microbials to safe levels as set by public health

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6
Q

What does it mean if something is septic?

A

It is contaminated with pathogenic microorganisms

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7
Q

What is aseptic?

A

Free of pathogenic microorganisms

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8
Q

What is degermination?

A

The mechanical removal of microorganisms

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9
Q

What form of microbial growth control is hand soap?

A

Degermination

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10
Q

What does the -cide ending mean?

A

Kill

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11
Q

What does the -stat ending mean?

A

Slow, inhibit

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12
Q

What does the -lytic ending mean?

A

Rupture of cell (kills most of the time

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13
Q

What 4 factors affect antimicrobial effectiveness?

A
  1. Concentration of microbes - less cells, more effective
  2. Environmental factors - temp., pH, pure or mixed culture
  3. Time of exposure
  4. Specific microbial characteristics - resistance to death
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14
Q

What is Decimal Reduction Time?

A

The time in which 90% of microbial population will be killed at a given temperature

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15
Q

If the temperature is low, what will happen to the Decimal Reduction Time (D)?

A

It will increase

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16
Q

What is an example of moist heat that discourages microbial growth?

A

Autoclave - uses water, heat, pressure

17
Q

What is an example of dry heat antimicrobial?

A

Oven - used in clinical setting for glassware and metal

18
Q

What is the lowest temperature at which all microorganisms are destroyed in 10 minutes called?

A

Thermal Death Point

19
Q

What is the Thermal Death Time?

A

The minimum amount of time at which all microorganisms are destroyed at a a given temperature

20
Q

What is the x-ray an example of?

A

Ionizing radiation

21
Q

What is ionizing radiation?

A

x-ray, gamma ray, electron beams; form hydroxyl free radicals

22
Q

What is non-ionizing radiation?q

A

UV, damages cell components, ex. sunburn, damage nucleic acids

23
Q

What is pasteurization?

A

not sterilizing, heat decreases number of microorganisms to a safe level using low heat

24
Q

What is the use of membrane filters to retain certain organisms then treat and destroy or lower cell numbers called?

A

Filtration

25
How do toxic chemicals work to combat microbial growth?
gasses - toxic and impede growth | antimicrobials - don't necessarily kill organisms, concern for environment because they are harmful to living things
26
What is dessication?
Dehydration of microbes
27
What form of temperature lowering is most harmful to microorganisms and why?
Slow-low drop, because it causes crystals to form in the cell
28
What is one way to dehydrate a microorganism?
Placing them in a hypertonic solution (osmotic pressure)
29
What is the name of any chemical substance used to prevent or treat a wide range of problems?
Chemotherapeutic agent
30
What form of medicine is made by a living organism?
Antibiotic
31
What is the name of the lowest concentration of chemotherapeutic agent or antimicrobial that will kill a microorganism?
Minimum Inhibitory Concentration (MIC)
32
What are 2 ways to evaluate antimicrobials?
1. Use dilution test | 2. Disk diffusion method
33
How does the Use Dilution Test work?
Metal rings are dipped in cultures, then soaked in antimicrobial for 10 minutes, rings are then placed on growth media and incubated; look for growth
34
How does the Disk Diffusion Method work?
Filter paper disks are soaked in anti-microbial and placed on petri dish of microorganisms, incubated, if it works there is a clear zone on the plate