Chapter 7 Flashcards
What is microscopy?
An instrument used to see objects that are too small to be seen by the naked eye
When were microscopes invented?
In 1590 and was further refined during the 1600s
When were cells first seen by Robert Hooke?
1665
What is a light microscope (LM)?
A light microscope uses focused light and lenses to magnify a specimen, usually a cell.
What is resolution?
A measure of the clarity of the image. The minimum distance between two points that can be separated and still be distinguished at different points.
What is magnification?
The ratio of an object’s image size to its actual size
What is contrast?
The difference in brightness between the light and dark areas of an image.
What is the electron microscope (EM)?
Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens.
What is the scanning electron microscope (SEM)?
A scanning electron microscope (SEM) scans a focused electron beam over a surface to create an image. The electrons in the beam interact with the sample, producing various signals that can be used to obtain information about the surface topography and composition.
What is a brightfield (Unstained specimen) (LM)?
Where light passes straight through the specimen unless the cell is naturally pigmented or artificially stained.
What is a brightfield (stained specimen) (LM)?
Staining with various dyes which enhances contrast. This requires that cells be fixed thereby killing them
What is a phase-contrast (LM)?
Variations in density within the specimen are amplified to enhance contrast in unstained cells. This is useful for living cells.
What is the differential interference contrast (LM)?
Similar to phase-contrast where optical modifications are used to exaggerate differences in density. This image appears almost 3D
What is fluorescence (LM)?
Where the locations of specific molecules in the cell can be revealed by labelling the molecules with fluorescent dyes or antibodies.
What is confocal (LM)?
Using a lazer an optical sectioning technique eliminates out-of-focus light from a thick sample creating a single plane of fluorescence in the image. This allows for a 3D construction to be formed.
What is deconvolution (LM)?
Images through deconvolution software which digitally removes out-of-focus light and re-assigns it to its source creating a much sharper 3D image.
What is super resolution (LM)?
Where sophisticated equipment is used to light up individual florescent molecules and record their position. Combining information from many molecules in different places breaks the limit of resolution resulting in a super sharp image.
What does LM stand for?
Light micrograph
What does SEM stand for?
Scanning electron micrograph
What does TEM stand for?
Transmission electron micrograph.
What is the transmission electron micrograph?
Used to study the internal structure of the cells. It aims an electron beam through a very thin section of the specimen allowing scientists to observe features such as structure.
What is cytology?
The study of cell structure
What is cell fractionation?
Used to seperate cell components based on size and density. It takes cells apart and separates major organelles and other sub cellular structures from one another.
What is the centrifuge?
A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g. cream from milk) or liquids from solids.