Chapter 6 Flashcards
helps researchers determine the amount of protein
Concentration assay
the amount & length of activity for a given protein in test organism
Potency assay
how long a pharmaceutical product is effective & how it should be stored
Stability assay
shows if a specific protein is present & conducting a specific reaction
Activity assay
highly specific test to determine protein concentration based on a particular antigen & antibody interaction including recognizing protein contaminants
testing for antigens
measuring amount of proteins
ELISA
pharmacokinetic and pharmacokinetic → understand the relationship between dosage & activity
PK/PD assays
used to determine safe & unsafe levels
Toxicology assay
a single antibody with a conjugated enzyme
Direct ELISA
use of a primary antibody and secondary
Indirect ELISA
mammalian cells incorporate recombinant DNA
Transfection
delivery of a drug
cream, inhaler, pill, etc.
Formulation
a test for determining the presence of a protein of interest
Assay
molecules produced by bacteria or fungus that inhibits bacterial growth
Antibiotic
early stages of product development → discovery of structure or function of a product and initial small scale reduction
Research and development
strict series of tests that evaluates effectiveness & safety in humans
Amylase
enzyme that breaks down amylose into maltose found in fungus
amylase
3 specific uses of amylase in the industry
soften paper
soften textiles
sweeten beverages
enzymes used as a reporter molecule in an ELISA or western blot
Horseradish peroxidase
process in which a gel that contains protein is transferred into a positively charged membrane
Western blot
a version of an ELISA that detects HCG from urine
pregnancy test
an assay for the presence of starch using iodine
Starch test
not subject to FDA approval; adverse consequences; certain combinations= bad
Herbal remedies
different shade of blue depending on different concentration of protein
bradford reagent
a place with great sources of plant and animal life that could be a source of new therapeutics
rainforests
CPDP 5 steps
does the product meet a critical need?
is the market large enough?
does preliminary data support safety & efficacy?
can you get patent protection?
can you make a profit?
