Chapter 3 Flashcards
Aqueous
solution where water is the solvent
Buffer
resists changes in pH
Concentration
proportion of solute to solvent
Dilution
addition of more solvent to make a solution less concentrated
Molarity
concentration of a solution expressed in a number of moles per solute per meter of solvent
Mole
molecular weight of a substance in grams
Solute
what gets dissolved in the solution
Solvent
liquids that does the dissolving
Micropipet
instrument used to measure very tiny volumes less than 1 mL
Pipet
instrument used to measure 0.1 ml and 50 ml
Multichannel pipet
type of micropipet hat holds 4 to 16 pipet tips
Graduated cylinder
plastic or glass tube equally spaced to show graduations
10 mL to 2 L
Tabletop balance
instrument measures mass
usually not less than 1 g
Analytical balance
less than 1 g
Meniscus
concave meniscus when a molecule attracted to the container vs. their own molecules
stick to the edges
Tap water
only used for washing glassware
Instructions for solution preparation
2 molar solution of this
weight it out
add to container
add water
pH measure
How to clean containers:
1.wash vessel with laboratory soap and water
2.rinse with tap water
3.then rise 5 more times
4.do a final rinse with deionized water
When do you use C1V1=C2V2 equation
when preparing diluted solutions
What is C1
concentration of concentrated solution (starting solution)
What is V1
volume of the stock solution in diluted sample
What is C2
desired concentration
WHat is V2
desired volume of sample
TRIS
organic molecule that is a buffer salt
TE buffer
stores DNA
TAE buffer
running DNA samples in agarose gels in horizontal gel box
