Chapter 6 Flashcards
What are all organisms made out of?
Cells
What are cells?
- The basic structural and functional unit of every organism
- The simplest collection of matter that can be alive
What is cell structure correlated to?
Cellular function
How are all cell structures related?
All cells are related by their descent from earlier cells
Scientists use what to visualize cells?
microscopes
What occurs in a light microscope?
-visible light is passed through a specimen and then through glass lenses
-the lenses refract(bend) the light, so that the image is magnified
What are the 3 important parameters of microscopy?
magnification, resolution, contrast
Magnification
the ratio of an object’s image size to its real size
Resolution
the measure of the clarity of the image or the minimum distance of 2 distinguishable points
Contrast
visible differences in parts of the sample
Light microscopes can magnify effectively to about
1,000 times the size of the actual specimen
Most sub-cellular structures, including organelles(membrane enclosed compartments) are too small to be resolved by
a light microscope
What are the 2 types of electron microscopes(EMs)?
Scanning electron microscopes and transmission electron microscopes
Scanning Electron Microscopes (SEMs)
focus on a beam of electrons onto the surface of a specimen, providing images that look 3D
Transmission Electron Microscopes (TEMs)
-focus on a beam of electrons through a specimen
-used mainly to study the internal structure of cells
What forms of microscopy provide sharper images of 3-dimensional tissues and cells?
Confocal microscopy and deconvolution microscopy
What is cell fractionation?
-process of taking cells apart and separating the major organelles from one another
-enables scientists to determine the functions of organelles
Centrifuges
fractionate cells into their component parts
What are the basic features of all cells(prokaryotes and eukaryotes)?
-plasma membrane
-semifluid substance called cytosol
-chromosomes(carry genes in the form of DNA)
-ribosomes(make proteins)
-ATP
Prokaryotic cells are characterized by having
-no nucleus
-DNA in an unbound region called the nucleoid
-no membrane-bound organelles
-cytoplasm bound by the plasma membrane
Eukaryotic cells are characterized by having
-DNA in a nucleus that is bounded by a membranous nuclear envelope
-membrane-bound organelles
-cytoplasm in the region between the plasma membrane and nucleus
-generally much larger than prokaryotic cells
Mycoplasmas
-the smallest bacteria
-1.0 um in diameter
What is the average size in diameter of most bacteria?
1-5 um
What is the average size of eukaryotic cells?
10-100 um in diame
As the surface area of a cell increases by a factor of n2
the volume increases by a factor of n3
Which cells have a greater surface area relative to volume?
small cells
What is the plasma membrane?
-selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell
What is the general structure of a biological membrane?
a double layer of phospholipids
What does the nucleus contain?
-most of the DNA in a eukaryotic
-most of the cell’s genes
-usually the most conspicuous organelle
What does the nuclear envelope do?
encloses the nucleus, separating it from the cytoplasm
What is the nuclear membrane made up of?
a double membrane; each membrane consists of a lipid bilayer
What do ribosomes do?
use information from the DNA to make proteins
Pores
regulate the entry and exit of molecules from the nucleus
Nuclear lamina
-composed of proteins
-maintains the shape of the nucleus
Chromosomes
composed of a single DNA molecule associated with proteins(in the nucleus)
Chromatin
-DNA and proteins of chromosomes
-condenses to form discrete chromosomes as a cell prepares to divide
What is the nucleolus?
-the site of ribosomal RNA (rRNA) synthesis
-located within the nucleus
Ribosomes
particles made up of ribosomal RNA and protein
Where do ribosomes carry out protein synthesis?
-in the cytosol(free ribosomes)
-on the outside of the endoplasmic reticulum or nuclear envelope(bound ribosomes)
What are the components of the endomembrane system?
-nuclear envelope
-endoplasmic reticulum (ER)
-golgi apparatus
-lysosomes
-vacuoles
-plasma membrane
-either continuous or connected via transfer by vesicles
What are the tasks of the endomembrane system?
-synthesis of proteins and their transport into membranes and organelles or out of the cell
-metabolism and movement of lipids
-detoxification of poisons
Endoplasmic reticulum (ER)
-accounts for more than half of the total membrane in many eukaryotic
-ER membrane is continuous with the nuclear envelope
What are the 2 distinct regions of the ER?
smooth ER, rough ER
Smooth ER
lacks ribosomes
Rough ER
surface is studded with ribosomes
What does the smooth ER do?
-synthesizes lipids
-metabolizes carbohydrates
-detoxifies drugs and poisons
-stores calcium ions (in muscles)
What does the rough ER do?
-bound ribosomes secrete glycoproteins
-distributes transport vesicles, proteins surrounded by membraned
-is a membrane factory for the cell
what are glycoproteins?
proteins covalently bonded to carbohydrates