Chapter 4 - Proteins

Question 1

Proteins as biotechnology products

Answer 1

beer brewing and winemaking, cheesemaking
recombinant DNA tech makes it possible to produce specific proteins on demand - enzymes, hormones, antibodies, structural proteins.

Some examples: Amylases, proteases (detergents, digestive aides), lipases, pectinases, lactases, etc)

Question 2

Proteins

Answer 2

complex molecules built of chains of amino acids. have specific molecular weights. have electrical charge

Question 3

Post-translational modifications

Answer 3

can affect proteins activity by increasing solubility, orienting proteins into membranes, and extending or decreasing active life of a molecule

Question 4

Protein engineering by directed molecular evolution

Answer 4

using directed molecular evolution technology to introduce specific predefined alterations in amino acid sequence

Question 5

Recombinant Proteins (rProteins)

Answer 5

genetically modified enzymes - more effective pH, temp, chemicals

Question 6

Upstream Processing

Answer 6

includes actual expression of the protein in the cell.

Question 7

Downstream processing

Answer 7

involves purification of the protein and verification of the function, a stable means of preserving the protein is required. Choices made during upstream can simplify downstream