Chapter 4 Flashcards
Rate of diffusion in cell affected
Surface area available
Temperature
Concentration gradient (quantity of molecules inside and out)
Distance
Cell radius increases
Surface area increases
Volume increases
10 times
100 times
1000 times
Resolution
Minimum distance two points can be apart and still distinguished
Microscope types
Light- visible light 200 nm
Transmission Electron microscope (TEM) - 0.2 nm Through material
Scanning electron microscope- (SEM) 0.2 nm surface of specimen
All cells have
Nucleoid or nucleus
Cytoplasm
Ribosomes
Plasma membrane
Nucleoid or nucleus
Nucleoid (prokaryote) Nucleus (Eukaryote) Genetic info
Cytoplasm
Semifluid matrix of organelles and cytosol
Ribosomes
Synthesize proteins
Plasma Membrane
Semi permeable barrier separating inside and outside
Types of chromosomes
(Prokaryote) Single circular chromosome (yarn like, not. a circle)
(Eukaryote) Multiple rod like chromosomes
Central Dogma of biology
DNA —> Transcription —> RNA —> translation —> Protein
Capsule
S layer- solid layer of carbs or slimy (glycocarics)
Bacterial cell wall
Peptidoglycan (pep-tido-gly-can)
Protection
Maintain cell shape
Prevents excessive H2O uptake
Flagella and 4 types
Monotrichous- single
Amphitrichous - two: one on each end
Lophotrichous - multiple coming off one end
Peritrichous - multiple flagella on all sides of cell
Pili
hairs on outside for movement or attachment
Archaea attributes and 4 main differences from bacteria
many are Extremophiles
1 chemical structure of plasma membrane (tails connect in phospholipid bilayer)
2 Cell wall, when present, lacks peptidoglycan
3 DNA replication more similar to eukaryotes
4 Protein production more similar to eukaryote
Compartmentalization
membrane bound organelles- goal is to make chemical reactions more favorable with smaller compartments
Cytoskelton
support and cell structure
Cytoplasm Components
Cytosol + Organelles (both euk and prokaryotes)
Cytosol
Region outside of organelles
Membrane-bound organelles
Nucleus and endomembrane system (endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes, and vacuoles)
Semi autonomous organelles
Mitochondria and Chloroplast
Nuclear envelope (membrane)
2 phospholipid bilayers
Nuclear pores
Nucleolus
region where ribosomes synthesis/assembly takes place
Chromatin
DNA plus associated histone proteins (wrapped to make smaller)
Ribosomal RNA
Large subunit and small subunit clamp to mRNA to direct protein synthesis
rRna
mRNA
tRNA
ribosomal RNA
messenger RNA
transfer RNA
Rough ER
Synthesis of proteins to be secreted, sent to lysosomes or plasma membrane, ribosomes attach to membrane
Endomembrane system
Internal membranes throughout cytoplasm. Cell material flows through system, proteins and lipids synthesized, modified, and transported
ER smooth and rough
Golgi apparatus
Lysosomes
Smooth ER
Makes lipids, stores calcium, detoxification
Golgi Apparatus
Package and distributed molecules (Proteins, lipids)
Cis vs trans face of Golgi
Cis faces ER
Trans faces away from ER
Vesicles
transport molecules to destination from Golgi
Transport vs Secretory vesicles
Phagocytosis
Taking in forgien matter or bacteria
Lysosomes
Membrane-bounded digestive vesicles
Come from Golgi apparatus
Have enzymes in lysosomes to breakdown macromolecules and phagocytosis
Vacuoles and types
(Plant cells, not animals) Membrane-bounded structure in plants
1 Central Vacuole - plant cells
2 Contractile vacuole - some fungi and protists - gets rid of water
3 Storage vacuoles
Central Vacuole and Turgor pressure
Water storage helps keep cell size and keeps plant from shrinking
Turgor pressure - Holding of water and plant shape
Mitochondria
Used for cell respiration (Energy, ATP, breaking bonds to create energy).
Bound by 2 membranes
Outer membrane
Intermembrane space
Inner membrane (has cristae [folds]) - has proteins that carry out oxidative metabolism
Matrix (inside inner membrane)
Have their own DNA (singular circular chromosomes) ribosomes, and division
Chloroplasts
Organelles in plants and some eukaryotes. Contains chlorophyll for photosynthesis
2 membranes
Thylakoids - membrane sacs within the inner membrane. Grana - stacks of multiple thylakoids
Stroma - fluid matrix inside chloroplast
Own DNA (singular circular) own replication DNA and ribsomes
Endosymbiosis
Proposes that some eukaryotic organelles evolved by a symbiosis of two free living cells
One prokaryote engulfed another and became part of cell
Cytoskeleton
Support shape, keep organelles in fixed locations, Dynamic system (constantly forming and disassembling)
3 types of Cytoskeleton fibers
Microfilaments
Microtubules
Intermediate filaments
Microfilaments
Smallest type Ex. Actin
Two protein chains loosely twined together
Movement like contraction, crawling, pinching. (Grab or release to move by pulling) String of pearls
Microtubules
Largest type. Dimers of alpha and beta tubulin subunits. Facilitate movement of cell and materials within cell. (Grab onto and move)
Intermediate filaments
Mid size. Very stable usually not broken down. Ex. Keratin (fingernails, hair, hooves)
Centrosomes
Packets where microtubules form perpendicular centrioles. Organize microtubules for cell division. (not all cells. almost all animal cells)
Flagella and Cilia (eukaryote)
9+2 arrangement of microtubules
Eukaryotic cell walls
Plant (2 walls) and protists- cellulose
Fungi-chitin
Animals- lack cell wall
Extracellular Matrix (ECM)
Secrete mixture of glycoproteins
Collagen may be abundant
Form protective layer over cell surface
Integrins link ECM to cell’s cytoskeleton (influences cell behavior)
Cell to cell interaction
Glycolipids - tissue specific cell surface marker
MHC protein - recognize self and nonself cells by the immune system
Tight junction
Connect the plasma membrane of adjacent cells in a sheet. No leakage
Anchoring junction
Mechanically attaches cytoskeleton of neighbor cells (desmosomes)
Communicating junction
Chemical or electrical signal passes directly from one cell to adjacent cell
Animal=Gap junction
Plant=plasmodesmata
Plasmodesmata
Special opening in cell walls where cytoplasm are connected with central tubule
Peroxisomes
Microbodies that contain enzymes used to oxidize fatty acids.