Chapter 4 Flashcards

1
Q

Simple staining

A

Single dye
Shape

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2
Q

Different staining

A

Two or more dyes
Gram staining

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3
Q

Special staining

A

Simple or diff stains used for specific microbial structures
Capsule stain endospore

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4
Q

Smaller wavelength

A

Better magnification

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5
Q

what does Refracts mean?

A

Bending

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6
Q

What is the formula to mesure light

A

550/2(n)

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7
Q

What is the purpose of staining?

A

Helps the bacteria to stand out

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8
Q

Resolution increases what?

A

Increase the magnification, the resolution improves but the revolving # gets smaller

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9
Q

what does Resolving power mean?

A

Number assigned to the quality
550/2(nm)

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10
Q

What is oil immersion used for?

A

Reduces light bending

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11
Q

Darkfield light

A

Visible light to observe colorless specimens against a black background

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12
Q

what is a Phase contrast light microscope?

A

Sees surface feature cells
Staining is not required
Living organisms can be observe

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13
Q

Fluorescences microscopy

A

Uses Uv light
Killed specimen

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14
Q

Electron microscopy

A

Provides 3d view

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15
Q

Principles of staining

A

Dyes are salts- cations +anions

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16
Q

the function of Direct staining?

A

Stains itself

17
Q

Indirect staining does what?

A

Stains the background

18
Q

What is the job of an endospore stain?

A

Highlights the presence of the endospore