Chapter 4 Flashcards
Simple staining
Single dye
Shape
Different staining
Two or more dyes
Gram staining
Special staining
Simple or diff stains used for specific microbial structures
Capsule stain endospore
Smaller wavelength
Better magnification
what does Refracts mean?
Bending
What is the formula to mesure light
550/2(n)
What is the purpose of staining?
Helps the bacteria to stand out
Resolution increases what?
Increase the magnification, the resolution improves but the revolving # gets smaller
what does Resolving power mean?
Number assigned to the quality
550/2(nm)
What is oil immersion used for?
Reduces light bending
Darkfield light
Visible light to observe colorless specimens against a black background
what is a Phase contrast light microscope?
Sees surface feature cells
Staining is not required
Living organisms can be observe
Fluorescences microscopy
Uses Uv light
Killed specimen
Electron microscopy
Provides 3d view
Principles of staining
Dyes are salts- cations +anions