Chapter 3: the Microscope

Question 1

guidelines for using a microscope

Answer 1

• transport it upright
• use only grit free lens paper to clean the lenses
• always begin focusing with the lowest-power objective lens in position; changing to higher power as necessary
• Use coarse adjustment knob only with the lowest poer lens
• use a coverslip with wet mount preparations

Question 2

Substage light

Answer 2

light passes directly upward through the microscope

Question 3

stage

Answer 3

the platform the slide rests on while being viewed

Question 4

condsensor

Answer 4

small substage lens that concentrates the light on the specimen.

Question 5

iris diaphragm lever

Answer 5

permits the best possible contrast when viewing

Question 6

coarse adjustment knob

Answer 6

used to focus on the specimen

Question 7

fine adjustment knob

Answer 7

used for precise focusing once coarse focusing has been completed.

Question 8

Ocular

Answer 8

eyepiece. ocular lens has a magnification of 10X

Question 9

objective lenses

Answer 9

adjustable lens system that permits the use of a scanning lens, a low-power lens, a high-power or oil immersion

Question 10

scanning lens

Answer 10

4X to 5X magnification

Question 11

low-power lens

Answer 11

10X magnification

Question 12

high-power objective lens

Answer 12

40X-50X magnification

Question 13

Oil immersion lens

Answer 13

100X mag

Question 14

real image

Answer 14

the objective lens magnifies the specimen to produce a real image that is projected to the ocular

Question 15

virtual image

Answer 15

the real image is magnified by the ocular lens to produce the virtual image

Question 16

total magnification

Answer 16

to get the total magnification multiply the power of the ocular lens by the power of the objective lens

Question 17

resolution

Answer 17

(resolving power) the ability to discriminate two close objects as seperate.

Question 18

depth of field

Answer 18

the thickness of the plane that is clearly in focus; greater at lower magnifications; as magnification increases, dept of field decreases

Question 19

field

Answer 19

the area you see through the microscope