Chapter 3: DNA Replication Flashcards

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1
Q

Semi-conservative??

A
  • one DNA strand from parental helix acts as the template

- one newly synthesized strand

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2
Q

dNTPs are added to the _’ end of the chain

A

3’ OH

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3
Q

DNA is synthesized _’ to _’

A

5’ to 3’

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4
Q

The template for DNA synthesis must be single or double stranded?

A
  • single!

- there must be a strand that extends on past the template

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5
Q

What are the primary replication enzymes?

A

Poly I and Poly III

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6
Q

there are __ nt/s in bacteria and - nt/s in humans?

A
  • 850

- 60-90

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7
Q

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaA?

A
  • initiator proteins bind.
    L> leads to the denaturation of DNA
    L> binds at 9-bp
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8
Q

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaB ?

A
  • helicase….binds to DNA with the help of DnaC
    L> break H-bonds btwn bases
    L>r 13-bp since its rich in A and T
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9
Q

Initiation of Replication:
- Replicator ( OriC in E.coli)
L> DnaG???

A

primase binds….its a DnaG gene product…. DNA polymerase binds

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10
Q

What are the three core enzymes of DNA Poly III?

A
  • DNA E
    DNA Q
    HOLE
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11
Q

What are the three enzymes of DNA poly I?

A
  • DNA E , DNA Q , DA e
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12
Q

Replication fork:

- The double stranded DNA section __ to reveal __ template strands

A
  • opens

ssDNA

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13
Q

Replication fork

- Leading strand?

A
  • 5-3 strand synthesized continuously
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14
Q

Replication fork

lagging strand?

A
  • 3-5’ strand synthesized discontinuously
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15
Q

Eukaryotic Polymerases?

- Pol aplha/primase?

A
  • initiates new strands in replication via primase

L> Poly a adds 10-20 nt of DNA

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16
Q

Eukaryotic Polymerases?

-Pol e - epsilon?

A
  • synthesize the leading strand
17
Q

Eukaryotic Polymerases?

- Poly delta?

A
  • synthesizes the lagging strand.
18
Q

Eukaryotic Replicons

- More than 1 ___ per chromosome.

A
  • replication

- bidirectional at each origin.