Chapter 3: Cell Structure And Function Flashcards

0
Q

In the early 20th century, the cell was envisioned as a…

A

Bag of soup full of floating ribosomes and enzymes

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1
Q

3 shared bacterial traits:

A
  1. Thick, complex outer envelope
  2. Compact genome
  3. Tightly coordinated cell functions
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2
Q

________ consists of a gel-like network

A

Cytoplasm

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3
Q

______ encloses the cytoplasm

A

The cell membrane

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4
Q

______ covers the cell membrane

A

Cell wall

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5
Q

What is the non-membrane bound area of the cytoplasm that contains the chromosome in the form of looped coils?

A

The nucleoid

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6
Q

External helical filament whose rotary motor propels the cell

A

Flagellum

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7
Q

What must first take place in order to isolate and study cell parts?

A

Cell fractionation

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8
Q

4 types of cell fractionation

A
  1. Mild detergent
  2. Sonication
  3. Enzymes
  4. Mechanical disruption
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9
Q

A key tool of subcellular fractionation is the….

A

Ultracentrifuge

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10
Q

What does centrifugation do?

A

Separates particles by size

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11
Q

Chemical components shared by all cells:

A
  1. Water
  2. Essential ions
  3. Small organic molecules
  4. Macromolecules
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12
Q

The structure that defines the existence of a cell is the…

A

Cell membrane

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13
Q

Membranes have approximately equal parts of…

A

Phospholipids and proteins

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14
Q

A phospholipid consists of…

A

Glycerol with ester links to two fatty acids and a phosphoryl head group

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15
Q

The two layers of phospholipids in the bilayer are called…

A

Leaflets

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16
Q

Membrane protein function:

A
  1. Structural support
  2. Detection of environmental signals
  3. Secretion of virulence factors and communication signals
  4. Ion transport and energy storage
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17
Q

Small molecules such as ___ and ___ easily permeate the membrane by _______.

A

O2; CO2; diffusion

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18
Q

Osmosis

A

The diffusion of water across a membrane

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19
Q

Protein transporters are needed to…

A

Transport polar and charged molecules across the cell membrane

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20
Q

Molecules move along their concentration gradient in

A

Passive transport

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21
Q

Molecules move against their concentration gradient in

A

Active transport

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22
Q

In eukaryotic membranes, the reinforcing agents are ______, such as ______.

A

Sterols; cholesterol

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23
Q

Hopanes/hopanoids

A

Membrane-reinforcing agents in bacteria

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24
Ether links between glycerol and fatty acids are in...
Archaea
25
In Archaea, hydrocarbon chains are branched...
Terpenoids
26
The most common structural support is the
Cell wall
27
The bacterial cell wall, or ______, consists of...
Sacculus; a single interlinked molecule
28
Most bacterial cell walls are made of...
Peptidoglycan (or murein)
29
Peptidoglycan consists of:
1. Long polymers of two disaccharides | 2. These are bound to peptides of 4 to 6 amino acids
30
Why are the enzymes responsible for peptidoglycan biosynthesis excellent targets for antibiotics?
Peptidoglycan is unique to bacteria
31
How does penicillin work?
It inhibits the transpeptidase that cross-links the peptides
32
How does vancomycin work?
It prevents cross-bridge formation by binding to the terminal D-Ala-D-Ala dipeptide
33
How does the widespread use of antibiotics effect microbes?
It selects for evolution of resistant strains
34
The _____-______ bacteria have multiple layers of peptidoglycan
Gram-positive
35
Composition of the capsule:
1. Polysaccharides | 2. Glycoproteins
36
Function of the capsule:
Protects cell from phagocytosis
37
Is the capsule found in gram-p or gram-n cells?
Both
38
An additional protective layer commonly found in free-living bacteria and archaea
S-layer
39
S-layer composition:
Crystalline layer of thick subunits consisting of protein or glycoprotein
40
How much peptidoglycan is in a gram-negative cell?
Thin layer of 1 or 2 sheets
41
What are lipopolysaccharides?
Lipids in the outer facing leaflet of gram-n cells that are toxic when released from the cell membrane
42
Where are the lipoproteins in gram-n bacteria?
On the inner leaflet
43
What does the outer membrane do in gram-n bacteria?
It confers defensive abilities and toxigenic properties on many pathogens
44
What is FtsZ?
A protein that gives bacteria the z-ring
45
MreB
Forms a coil inside rod-shaped cells
46
CreS
"Crescentin" - forms a polymer along the inner side of a crescent-shaped bactera
47
In bact. that appear superficially symmetrical, polar differences may appear at...
Cell division
48
Extensively folded intracellular membranes used for photosynthesis
Thylakoids
49
Bacterial structures that help control buoyancy
Gas vesicles
50
Carboxysomes
Polyhedral bodies packed with the enzyme Rubisco for CO2 fixation
51
What do bacteria use sulfur for?
Oxidation
52
What are glycogen, phb, and pha used for and where are they stored?
Energy; in the storage granules
53
Orient the swimming of magnetotactic bacteria
Magnetosomes
54
Straight filaments of pilin protein
Pili or fimbriae
55
Membrane-embedded extensions of the cytoplasm
Stalks
56
What do stalks do?
Secrete adhesion factors called holdfasts
57
Intercellular connections that pass material from one cell to the next
Nanotubes
58
The type of cell that has flagella randomly distributed
Peritrichous
59
Have a single flagella
Monotrichous
60
Have flagella at the ends
Lophotrichous
61
Each flagellum is a spiral filament of protein monomers called..
Flagellin
62
The flagellum is rotated by a motor driven by...
The proton motive force
63
The movement of a bacteria in response to chemical gradients
Chemotaxis
64
Repellents cause ____ rotation
CW
65
Attractants cause _____ rotation
CCW
66
Alternating runs and tumbles cause a
Random walk