Chapter 3: Bacterial Cell Structure Flashcards

1
Q

• Bacteria are referred to as → prokaryotes

A

• Lack a membrane-bound nucleus, membrane-bound
organelles, and internal membranous structures

The problem is some bacterial groups do have their genetic material enclosed in a → membrane
• Some also have membrane-bound → organelles

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2
Q

Most common shapes ___ and ___:

A

cocci ; rods

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3
Q

Cocci

A

spherical cells, single cells or clusters

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4
Q

Rod (bacilli)

A

cylindrical shape

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5
Q

Less common cells shapes are

A

Spirilla, Spirochetes, Vibrio, and Pleomorphic.

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6
Q

Spirilla

A

rigid, spiral-shaped cells

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7
Q

Spirochetes

A

flexible, spiral-shaped cells

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8
Q

Vibrio

A

resemble rods, comma
shaped

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9
Q

Pleomorphic

A

organisms that are
variable in shape

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10
Q

Arrangement of cells is determined by what?

A

• Determined by plane of division
• Determined by whether or not there is cell →
separation

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11
Q

Diplococci

A

pairs

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12
Q

Streptococci

A

chains

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13
Q

Staphylococci

A

grape-like clusters

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14
Q

Tetrads

A

4 cocci in a square

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15
Q

Sarcinae

A

cubic configuration of 8 cocci

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16
Q

Common features of a bacterial cell

A

• Cell envelope - 3 layers: cell membrane, cell wall, capsule
• Cytoplasm – internal fluid
• External structures

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17
Q

Bacterial Cell Envelope

A

• Cell envelope – plasma membrane and all of its external
surrounding layers

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18
Q

Bacterial plasma membrane

A

encloses the cytoplasm and
defines the cell’s → shape
• It is a selectively permeable barrier
• Only allows certain ions and molecules to enter or
exit and prevents others

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19
Q

Fluid-mosaic model

A

states that membranes consists of
fluid lipids with floating proteins

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20
Q

Plasma Membrane Structure

A

• Lipids in the membrane are →
phospholipids
• Amphipathic – both polar and
nonpolar ends
• Hydrophilic – polar phosphate
ends interact with → water
• Hydrophobic – nonpolar fatty
acid ends are insoluble in water
and only associate with → one
another

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21
Q

Amphipathic

A

both polar and
nonpolar ends

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22
Q

Hydrophilic

A

polar phosphate
ends interact with → water

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23
Q

Hydrophobic

A

nonpolar fatty
acid ends are insoluble in water
and only associate with → one
another

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24
Q

Peripheral proteins

A

connected
to the membrane and are easily
removed

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25
Q

Integral proteins

A

transport
proteins that move materials into
and out of the cells

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26
Q

Bacterial membranes lack

A

the sterol cholesterol

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27
Q

Bacterial membranes contain hopanoids that are….

A

Similar to cholesterol and probably stabilizes the membrane

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28
Q

Plasma membrane function as a barrier but also

A

have to
bring nutrients into the cell

Primary function is to obtain energy and nutrients
sources for the cell

Bacteria can only take in dissolved molecules

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29
Q

Bacteria mainly transport nutrients into the cell ____
their concentration gradient

A

against

• High levels in the cells (storage) and low levels in the
environment
• Bacteria commonly live in nutrient-poor habitats

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30
Q

Passive diffusion

A

movement of molecules from an area of high concentration to one with a low concentration
• Molecules move down the concentration gradient
• A large concentration gradient is required for adequate
nutrient uptake

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31
Q

___
, ____
, and, ___ cross the membrane by passive diffusion

A

• O2
, CO2
, and, H2O

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32
Q

Facilitated diffusion

A

substances move across the plasma
membrane with the help of a carrier or channel protein

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32
Q

Facilitated diffusion

A

substances move across the plasma
membrane with the help of a carrier or channel protein

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33
Q

Channel protein

A

pore, substances pass through

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34
Q

Carrier protein

A

carry substances across the membrane

• Carrier proteins change their shape to facilitate the
movement of a substance across the membrane

• Go back to their original shape when transport is →
completed

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35
Q

Active transport

A

substance moves from an area of a low concentration to a high concentration with the help of → energy

• Movement against a concentration gradient

• Uses carrier proteins and some form of energy to drive the reaction

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36
Q

Primary active transport

A

uses ATP
energy to move substances against a concentration gradient without
modifying the substance

Uses a uniporter

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37
Q

uniporter

A

moves a single
molecule across the membrane
• Ex. ABC Transporter

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38
Q

Secondary active transport

A

moves
substances against a concentration
gradient with the use of ion gradient →potential energy

• Uses cotransporters, which move two
substances at the same time

• The ion that powers the transport and the substance being moved across the membrane

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39
Q

Symporter (In secondary active transport)

A

ion and substance move in the same direction

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40
Q

Antiporter (In secondary active transport)

A

ion and substance move in opposite directions

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41
Q

Group translocation

A

molecule is chemically modified as
it is transported into the cell

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42
Q

Transporters of different types of sugars _____

A

phosphorylate
them (add a phosphate)

43
Q

Group translocation used by bacteria to _____

A

extract energy from organic energy
sources to generate ATP

44
Q

Cell wall

A

layer found outside of the plasma membrane

• Helps maintain cells shape and protects it from osmoticlysis (plasmoptysis)

• Protects the cell from → toxins

45
Q

Peptidoglycan

A

Rigid structure lying just outside the cell plasma
membrane

46
Q

Two types based on Gram stain

A

Gram-positive (monoderms)

Gram-negative (diderms)

47
Q

Gram-positive (monoderms)

A

stain purple; thick
peptidoglycan

48
Q

Gram-negative (diderms)

A

stain pink or red; thin
peptidoglycan and outer membrane

49
Q

Peptidoglycan
Structure

A

Meshlike polymer of identical
subunits forming long strands • Two alternating sugars • N-acetylglucosamine (NAG) • N- acetylmuramic acid (NAM) • Alternating → amino acids

50
Q

Peptidoglycan strands have a ___
shape

A

helical

51
Q

Peptidoglycan chains are _____ by peptides for _____

A

crosslinked; strength

• Called a peptide → interbridge
• Various structures occur

52
Q

Gram-positive bacteria

A

have thick cell walls made of
peptidoglycan and other polymers like → teichoic acids

53
Q

Teichoic acids

A

– polymers of glycerol or ribitol joined by
phospholipids (negatively charged)
• Covalently linked to → peptidoglycan

54
Q

Typical Gram-Positive Cell Walls
Consist Primarily of _____

A

Peptidoglycan

55
Q

Peptidoglycan

A

Create and maintain the structure of the cell envelope by
anchoring the cell wall to the plasma membrane
• Function to take-up → ions
• Help pathogenic species bind to host tissues

56
Q

Gram-negative

A

cell walls are more
complex then gram-positive
• Consist of a thin layer of
peptidoglycan surrounded by an
outer membrane
• Outer membrane composed of
lipids, lipoproteins, and →
lipopolysaccharide (LPS)
• No teichoic acids

57
Q

Gram-Negative Cell Walls

A

• The outer membrane of gram-negative bacteria lies outside of
the thin peptidoglycan layer
• Linked by lipoproteins
• Most abundant protein
linked to underlying
peptidoglycan and
embedded in the outer
membrane
• Outer membrane also contains
lipopolysaccharides (LPSs) – consist of both a
lipid and carbohydrates

58
Q

lipopolysaccharides (LPSs)

A

consist of both a
lipid and carbohydrates

59
Q

Functions of Gram-Negative Cell

A

• Contribute to the cells walls negative charge
• Stabilize the outer membrane and create a
permeability barrier
• Helps protect pathogenic bacteria from → host
defenses (O antigen)
• Acts as a toxin called endotoxin (lipid A) – causes
septic shock once it enters the bloodstream

60
Q

Hypotonic environments

A

• Solute concentration outside cell less than inside cell
• Water moves into cell and cell swells
• Cell wall protects from → lysis (plasmoptysis)

61
Q

Hypertonic environments

A

• Solute concentration outside cell is greater than inside
• Water leaves the cell
• Plasmolysis (plasma membrane shrinkage)occur

62
Q

Lysozymes

A

(antimicrobial in tears, saliva, and oil) breaks
bond between → NAG and NAM

63
Q

Penicillin inhibits

A

peptidoglycan synthesis
• If cells are treated, they lyse in a hypotonic solution

64
Q

Protoplasts

A

bacteria that have had the → cell wall
removed

65
Q

Bacteria that Lack Cell Walls

A

Mycoplasma
• Never produce a cell wall
• Plasma membrane more resistant to osmotic pressure

66
Q

Capsules

A

composed of polysaccharides, well organized,
and → not easily removed

Capsules help pathogenic bacteria resist phagocytosis by
host phagocytes
• Found outside of the cell wall
• When bacterial cells that lack capsules they are
phagocytized easily and do not cause disease
• When capsulated they can quickly cause disease

67
Q

Slime layer

A

Functions in attachment of bacteria to solid surfaces
and host tissue surfaces (biofilms)

• made of unorganized polysaccharides that
are → easily removed
• Produced by gliding bacteria to facilitate → movement
• Slime layers and capsules consist of glycocalyx – network
of polysaccharides coming off of the cell surface

68
Q

Protoplast

A

plasma membrane and everything contained
→ within

69
Q

Cytoplasm

A

the material contained within the plasma
membrane
(cytosol, ribosomes, and plasmids)

70
Q

cytosol

A

the liquid component

71
Q

Bacterial Cytoskeleton

A

Homologs of all 3 eukaryotic cytoskeletal elements have
been identified in bacteria

• Functions are similar as in eukaryotes
• Participate in → cell division
• Localize proteins
• Determine → cell shape

72
Q

Inclusions

A

formed by the clumping of substances
• Granules, crystals, or globules of organic or inorganic
material that are stored by the cell for → future use

73
Q

Storage inclusions are formed when a
nutrient has a….

A

good supply, and another →
does not

• End products of metabolic reactions are
commonly stored and used later used by
the microbe when it is in a harsh
environmental condition

74
Q

Microcompartments enclose one or more

A

enzymes

75
Q

Carboxysomes

A

present in
cyanobacteria (photosynthetic bacteria)
• Contain enzymes of photosynthesis
reactions
• Microcompartment prevents CO2
from escaping so it can be
converted into → sugars

76
Q

Gas vacuoles

A

allow aquatic,
photosynthetic bacteria to be→
buoyant
• Made up of clumped→ gas vesicles

77
Q

Microbes can adjust their depth to
reach proper

A

light, oxygen
concentrations, and nutrient levels
• Descend by collapsing vesicles and
ascend by forming → new ones

78
Q

Magnetosomes

A

formed by magnetotactic
bacteria that orient along → magnetic fields
• Intracellular chains of magnetite or greigites
particles (iron ores) enclosed within
invaginations of the plasma membrane
• Act as tiny magnets to swim to nutrient-rich
sediments or locate fresh or marine water
habitats

79
Q

Ribosomes

A

site of → proteins synthesis
• Found in the cytoplasm or attached to the plasma
membrane

80
Q

• Bacterial ribosomal RNA =

A

70S
• 16S small subunit
• 23S and 5S in large subunit

81
Q

Nucleoid

A

contains the cell’s chromosomes and several
proteins
• Most bacterial have a single circular chromosome of
DNA, some have a linear chromosome, and some have
more than one chromosome

82
Q

Binary fission

A

asexual reproduction in bacteria

Single circular chromosome → replicates

Plasma membrane and cell wall separate
the cell into → two identical cells

83
Q

Plasmids

A

extrachromosomal DNA

• Found in bacteria, archaea, some fungi
• Usually small, closed circular DNA molecules
• Exist and replicate independently of chromosome

84
Q

Episomes

A

may integrate into chromosome
• Inherited during → cell division

85
Q

Fimbriae or pili

A

fine, hair-like
structures that are thin and short

86
Q

Fimbria

A

function to attach cells
to solid surfaces like host tissue or
rocks in streams

87
Q

Sex pili

A

involved in motility and
gene transfer
• Conjugation

88
Q

Flagella

A

used by many motile bacteria
• Threadlike appendage that extends outward from the
plasma membrane and cell wall
• Main function is → motility

89
Q

Flagellar Movement

A

Flagellum rotates like a propeller
• Very rapid rotation up to 1100
revolutions/sec
• In general, counterclockwise (CCW)
rotation causes forward motion (run)
• In general, clockwise rotation (CW)
disrupts run causing cell to stop and
tumble

90
Q

Swarming

A

• Occurs on moist surfaces as a type of
group behavior by bacteria
• Most swarmers have peritrichous
(uniform) flagella
• Production of molecules that aid
movement is typical

91
Q

Spirochete Motility

A

• Multiple flagella form axial fibril
which winds around the cell
• Flagella remain in periplasmic space
inside outer sheath
• Corkscrew shape exhibits flexing and
spinning movements

92
Q

Twitching

A

• Pili at ends of cell
• Short, intermittent, jerky motions
• Cells are in contact with each other and the surface

93
Q

Gliding

A

• Smooth movements (slime layer)

94
Q

Chemotaxis

A

the movement toward or away from
chemical attractants or repellants
• Move in response to temperature, light, oxygen,
osmotic pressure, and gravity

95
Q

Phototaxis

A

movement of a motile
organism in response to light,
either toward the source of light or
away from it
• Common with → cyanobacteria

96
Q

Endospores

A

dormant cells formed within mother cells
• Resistant to environmental stresses like heat, ultraviolet
radiation, gamma radiation, chemical disinfectants, and
desiccation

97
Q

Clostridium botulinum

A

Example of endospore.

causes botulism toxin, food-borne disease
• Food must be prepared and stored properly

98
Q

Bacillus anthracis

A

Example of endospore.

causes anthrax if inhaled , spores
germinate in the lungs

99
Q

Endospore forming bacterial are commonly found in

A

soil

100
Q

Sporulation

A

process of making endospores.

growth ceases due to a → lack of
nutrients
• Produces a dormant cells that can survive until
nutrients are available and vegetative growth will
restart

101
Q

Formation of Vegetative Cell

A

• Activation

• Germination

• Outgrowth

102
Q

Activation

A

• Prepares endospores
for germination
• Often results from
treatments like →
heating

103
Q

Germination

A

• Environmental
nutrients are detected
• Spore swelling and
rupture or absorption
of spore coat
• Increased metabolic
activity

104
Q

Outgrowth

A

• Emergence of
vegetative cell