chapter 3

Question 1

what do you need for a polymerase chain reaction?

Answer 1

• DNA
• taq polymerase
• free nucleotide bases
• DNA primers

Question 2

what does polymerase chain reactions do and why would you use it

Answer 2

AMPLIFIES (duplicates) a DNA sequence. can be used when detecting a specific gene sequence eg in forensics

Question 3

different types of primers

Answer 3

forward primer - goes to 3’end of template strand

reverse primer - goes to 3’end of coding strand

Question 4

names and temperatures of the stages of polymerase chain reaction

Answer 4

1. denaturation 90-95*c
2. annealing 50-55*c
3. extension 72*c

Question 5

describe the process of gel electrophoresis

Answer 5

1. dna fragments are inserted into wells in the AGAROSE GEL
2. they travel to the positive ends, helped by BUFFER SOLUTION
3. smaller pieces travel faster and they all spread out. now you can compare the pieces.