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MBIO > Chapter 3 > Flashcards

Chapter 3 Flashcards

1
Q

What is a light microscope ?

A

Any kind of microscope that uses visible light to observe a specimen

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2
Q

What are the types of lens that light microscope use to magnify the image

A

objective lens
ocular lens

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3
Q

What is the range of magnification of the objective lens ?

A

10x -100x

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4
Q

What is the range of magnification of the ocular lens ?

A

10x

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5
Q

How to calculate total magnification using light microscope ?

A

Objective lens x Ocular lens

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6
Q

What is resolution ?

A

It is the ability to distinguish fine detail
and structure

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7
Q

What is resolution general principle ?

A

the shorter the wavelength, the
better the resolution

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8
Q

Between the light and electron microscope which one has a greater resolving power

A

Electron microscope

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9
Q

What is the range of magnification of electron microscope ?

A

up to 500 000 X

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10
Q

What is the function of an electron microscope ?

A

Allows us to view viruses
and internal cell structures.

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11
Q

What are the types of electron microscope ?

A

Transmission(TEM)
Scanning (SEM)

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12
Q

Which electron microscope is less powerful ?

A

Scanning0

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13
Q

How far can a human eye sees ?

A

0.2mm

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14
Q

How far can a light microscope view an object ?

A

0.2 micrometer

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15
Q

How far can a electron microscope view an object ?

A

2 nm

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16
Q

What are the named of positively and negatively charged ions in stains ?

A

chromophore

17
Q

Positive stains

A

Stain will bind to the bacterium
• Bacterium appears colored
• Background appears clear
• Ex. Crystal violet

18
Q

Negative stain

A

Will not bind to the bacterium
• Bacterium appears clear
• Background is colored
• Ex. nigrosin.

19
Q

What is function of spore stain ?

A

stains an internal structure of some bacteria

20
Q

What is the primary stain colour of spore stain ?

A

endospores green

21
Q

What is the function of the flagella stain ?

A

Stains an external structure

22
Q

What is the function of the acid fast stain ?

A

Detects the presence of a waxy compound in cell wall

23
Q

What is the function of the capsule stain ?

A

Detects a thick layer of polysaccharide outside the cell – capsule

MBIO (3 decks)

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